CAJ | 학술논문

目的：探讨吡格列酮在放射性肺纤维化中的保护作用,以及对AT1R(血管紧张素Ⅱ1型受体表达的影响。方法 SD大鼠60只,共分为5组。空白组：不接受任何干预；照射+安慰剂组：接受12 Gy照射+安慰剂干预；单独给药组：接受吡格列酮10 mg/( kg·d)干预；照射+吡格列酮 a组：接受12 Gy照射+吡格列酮10 mg/( kg·d)干预；照射+吡格列酮b组：接受12 Gy照射+吡格列酮20 mg/( kg·d)干预。采用HE及Masson染色观察大鼠肺组织纤维化情况；采用免疫组化观察AT1R在肺组织中的分布情况；采用蛋白免疫印迹杂交及RT-PCR检测AT1R蛋白及mRNA的表达情况。结果 HE及Masson染色显示,受照射大鼠肺组织细胞出现变性、坏死,肺组织出现明显纤维化；大鼠给予吡格列酮干预后,受照射大鼠的肺组织损伤减轻,肺组织纤维化减轻；免疫组化提示,照射后大鼠肺组织及上皮细胞中AT1R表达较空白组及单独给药组明显,照射+吡格列酮组的AT1R表达较照射+安慰剂组少；Western blot及RT-PCR提示,接受吡格列酮干预的受照射大鼠,AT1R蛋白及mRNA表达水平被明显抑制。结论吡格列酮在放射性肺纤维化中具有保护作用,其可能通过下调AT1的表达来实现对放射性肺损伤的保护作用。
목적：탐토필격렬동재방사성폐섬유화중적보호작용,이급대AT1R(혈관긴장소Ⅱ1형수체표체적영향。방법 SD대서60지,공분위5조。공백조：불접수임하간예；조사+안위제조：접수12 Gy조사+안위제간예；단독급약조：접수필격렬동10 mg/( kg·d)간예；조사+필격렬동 a조：접수12 Gy조사+필격렬동10 mg/( kg·d)간예；조사+필격렬동b조：접수12 Gy조사+필격렬동20 mg/( kg·d)간예。채용HE급Masson염색관찰대서폐조직섬유화정황；채용면역조화관찰AT1R재폐조직중적분포정황；채용단백면역인적잡교급RT-PCR검측AT1R단백급mRNA적표체정황。결과 HE급Masson염색현시,수조사대서폐조직세포출현변성、배사,폐조직출현명현섬유화；대서급여필격렬동간예후,수조사대서적폐조직손상감경,폐조직섬유화감경；면역조화제시,조사후대서폐조직급상피세포중AT1R표체교공백조급단독급약조명현,조사+필격렬동조적AT1R표체교조사+안위제조소；Western blot급RT-PCR제시,접수필격렬동간예적수조사대서,AT1R단백급mRNA표체수평피명현억제。결론필격렬동재방사성폐섬유화중구유보호작용,기가능통과하조AT1적표체래실현대방사성폐손상적보호작용。
Objective To investigate the protective effects of pioglitazone on radiation induced lung fibrosis, and the effects on the expression of AT1 receptor. Methods Sixty SD rats were divided into 5 groups. The blank group:without any intervention. The irradiation plus placebo group:receiving 12 Gy irradiation plus placebo interven-tion. The single drug group:receiving only 10 mg/( kg·d) pioglitazone intervention. The irradiation plus pioglitazone a group:receiving 12 Gy irradiation plus 10 mg/( kg·d ) pioglitazone intervention. The irradiation plus pioglitazone b group:receiving 12 Gy irradiation plus 20 mg/( kg·d) pioglitazone intervention. HE and Masson staining were used to observe the lung tissue injury. The distributions of AT1R in lung tissue were observed by immunohistochemistry. The expression of AT1R protein and mRNA were detected by Western blot and RT-PCR. Results HE and Masson staining indicated that the lung tissue of irradiation rats showed degeneration and necrosis,the lung tissue appeared obvious fi-brosis. When the rats were treated with pioglitazone after irradiation,the lung injury and fibrosis decreased. The immu-nohistochemical staining showed that after irradiation,the expressions of AT1R of lung tissue was more obvious than those of blank group and the single drug group. The expressions of AT1R of irradiation plus pioglitazone group was less obvious than that of irradiation plus placebo group. The Western blot and RT-PCR indicated that after the pioglitazone intervention,the expressions of AT1 protein and mRNA of irradiation rats were significantly inhibited. Conclusion Pi-oglitazone has protective effects on radiation induced lung fibrosis,possibly through the down-regulation of the expres-sion of AT1R.