CAJ | 학술논문

目的：研究阿尔茨海默病（AD）动物模型APP/PS1双转基因小鼠基因组DNA甲基化分布。方法采用最新发展的甲基化DNA免疫共沉淀（MeDIP）结合高通量测序方法，检测APP/PS1双转基因小鼠皮层脑组织DNA甲基化分布。结果只在AD小鼠脑组织中存在的DNA甲基化片段有2346个，涉及485个基因，这些DNA甲基化片段分布在不同的染色体上。部分甲基化基因具有一定家族聚集性。结论 APP/PS1双转基因小鼠与相应的野生型小鼠脑组织的DNA甲基化位点存在明显差异，提示DNA甲基化可能参与了AD的发生和发展。
목적：연구아이자해묵병（AD）동물모형APP/PS1쌍전기인소서기인조DNA갑기화분포。방법채용최신발전적갑기화DNA면역공침정（MeDIP）결합고통량측서방법，검측APP/PS1쌍전기인소서피층뇌조직DNA갑기화분포。결과지재AD소서뇌조직중존재적DNA갑기화편단유2346개，섭급485개기인，저사DNA갑기화편단분포재불동적염색체상。부분갑기화기인구유일정가족취집성。결론 APP/PS1쌍전기인소서여상응적야생형소서뇌조직적DNA갑기화위점존재명현차이，제시DNA갑기화가능삼여료AD적발생화발전。
Objective To investigate the distribution pattern of genome?wide CpG methylation in the animal model of APP/PS1 transgenic mice with Alzheimer′s disease(AD). Methods This study investigated the genome?wide DNA methylation profiles in the cortex tissues using methylat?ed DNA immunoprecipitation(MeDIP)combined with high?throughput sequencing. Results The analysis revealed 2 346 CpG sites existed only in AD mice,representing 485 unique genes as potentially associated with AD and these methylated DNA fragments distributed in different chromo?somes. Some hyper?methylated genes displayed familial aggregation. Conclusion There is significant difference in DNA methylation sites between APP/PS1 transgenic AD mice and corresponding wild mice,suggesting that DNA methylation may be involved in onset and development of AD.