中国医科大学学报
中國醫科大學學報
중국의과대학학보
JOURNAL OF CHINA MEDICAL UNIVERSITY
2015年
2期
148-151,159
,共5页
刘学勇%张展%邓纯博%付勤
劉學勇%張展%鄧純博%付勤
류학용%장전%산순박%부근
腰椎管狭窄%诱导性一氧化氮合酶%凋亡
腰椎管狹窄%誘導性一氧化氮閤酶%凋亡
요추관협착%유도성일양화담합매%조망
lumbar spinal canal stenosis%inducible nitric oxide synthase%apoptosis
目的:研究大鼠马尾神经慢性受压后诱导型一氧化氮合酶(iNOS)在脊髓内的变化,及其与相应节段脊髓内神经细胞凋亡的相关性。方法将健康成年SD大鼠30只随机分为对照组和实验组。对照组行假手术,仅切除L5椎板,实验组在L4平面置入硅胶片,造成马尾神经单节段受压。实验组分别于建模后第2、4、8、12周后,对照组于第4周,取腰L4前角脊髓组织,切片后进行免疫组织化学方法并结合图像分析技术,检测脊髓内iNOS的表达变化,应用TUNEL法检测凋亡神经细胞,透射电镜观察神经细胞形态学改变。结果对照组脊髓有极少量iNOS表达;实验组造模后第4周有轻度表达,第8、12周iNOS明显高于对照组。对照组脊髓中可见少量神经细胞凋亡,实验组从第4周起可见明显凋亡的神经细胞,且凋亡数量随时间的延长而增多。透射电镜观察发现实验组神经细胞发生凋亡改变。结论大鼠马尾神经慢性受压后,相应节段脊髓神经细胞iNOS表达增加,神经细胞发生凋亡,iNOS与神经细胞凋亡存在正相关。
目的:研究大鼠馬尾神經慢性受壓後誘導型一氧化氮閤酶(iNOS)在脊髓內的變化,及其與相應節段脊髓內神經細胞凋亡的相關性。方法將健康成年SD大鼠30隻隨機分為對照組和實驗組。對照組行假手術,僅切除L5椎闆,實驗組在L4平麵置入硅膠片,造成馬尾神經單節段受壓。實驗組分彆于建模後第2、4、8、12週後,對照組于第4週,取腰L4前角脊髓組織,切片後進行免疫組織化學方法併結閤圖像分析技術,檢測脊髓內iNOS的錶達變化,應用TUNEL法檢測凋亡神經細胞,透射電鏡觀察神經細胞形態學改變。結果對照組脊髓有極少量iNOS錶達;實驗組造模後第4週有輕度錶達,第8、12週iNOS明顯高于對照組。對照組脊髓中可見少量神經細胞凋亡,實驗組從第4週起可見明顯凋亡的神經細胞,且凋亡數量隨時間的延長而增多。透射電鏡觀察髮現實驗組神經細胞髮生凋亡改變。結論大鼠馬尾神經慢性受壓後,相應節段脊髓神經細胞iNOS錶達增加,神經細胞髮生凋亡,iNOS與神經細胞凋亡存在正相關。
목적:연구대서마미신경만성수압후유도형일양화담합매(iNOS)재척수내적변화,급기여상응절단척수내신경세포조망적상관성。방법장건강성년SD대서30지수궤분위대조조화실험조。대조조행가수술,부절제L5추판,실험조재L4평면치입규효편,조성마미신경단절단수압。실험조분별우건모후제2、4、8、12주후,대조조우제4주,취요L4전각척수조직,절편후진행면역조직화학방법병결합도상분석기술,검측척수내iNOS적표체변화,응용TUNEL법검측조망신경세포,투사전경관찰신경세포형태학개변。결과대조조척수유겁소량iNOS표체;실험조조모후제4주유경도표체,제8、12주iNOS명현고우대조조。대조조척수중가견소량신경세포조망,실험조종제4주기가견명현조망적신경세포,차조망수량수시간적연장이증다。투사전경관찰발현실험조신경세포발생조망개변。결론대서마미신경만성수압후,상응절단척수신경세포iNOS표체증가,신경세포발생조망,iNOS여신경세포조망존재정상관。
Objective To study the relationship between the expression of inducible nitric oxide synthase(iNOS)and neural cell apoptosis after chronic cauda equina compression. Methods Totally 30 male adult SD rats were randomly divided into 2 groups as the control group and the experi?mental group. The control group received sham operation with single laminectomy of L5 lumina. In the experimental group,the silicon sheet was in?serted into the spinal canal of L4 to cause single level compression of cauda equina. The L4 level of spinal cords were harvested at 2 weeks,4 weeks,8 weeks,and 12 weeks after operation in the experimental group,and at 4 weeks in the control group respectively,and then immunohistochemistry and image analysis were performed to observe the expression of iNOS in spinal cord and the TUNEL method was applied to observe cell apoptosis. The morphology of cells was observed by transmission electron microscope. Results There was few amount of iNOS expressed in the control group. The expression of iNOS was slight at 4 weeks in the experimental group and was higher at 8 weeks and 12 weeks compared with the control group. Small amount of neural cell apoptosis was evidenced in the control group,while neuron apoptosis appeared remarkably in the experimental group since 4 weeks and increased with the extension of time. Transmission electron microscopy found apoptosis changes in neurons in the experimental group. Conclusion The expression of iNOS increases in corresponding spinal cords after chronic compression of cuada equine and neural cell apoptosis oc?curs,indicating that iNOS is positively correlated with neural cell apoptosis.