CAJ | 학술논문

目的：探讨大鼠妊娠前后吸入异氟烷或七氟烷麻醉对子代大鼠脑发育的影响。方法将30只雌性SD大鼠随机分为5组（每组6只）：对照组（C组）、妊娠前1 d吸入1.6%异氟烷6 h组（BI组）、妊娠后6、10、14和18 d时吸入1.6%异氟烷6 h组（PI组）、妊娠前1 d吸入2.4%七氟烷6 h组（BS组）及妊娠后6、10、14和18 d吸入2.4%七氟烷6 h组（PS组）。于出生当天（T1）、出生后第7（T2）、14（T3）和28天（T4）时各组按窝别随机取12只子鼠处死取海马，HE染色法观察病理学改变，电镜下观察海马细胞超微结构，免疫组织化学染色法检测caspase?3、神经生长相关蛋白43（GAP?43）和神经型一氧化氮合酶（nNOS）的表达。结果与C组相比，BI组和BS组各时点、PI组和PS组T4时点子代大鼠海马中caspase?3、GAP?43和nNOS的表达差异无统计学意义（P>0.05），HE染色及电镜观察海马组织未见差异。PI组和PS组T1~T3时caspase?3表达增加，GAP?43和nNOS表达减少（P<0.01）， PI组HE染色海马结构有较明显的病理改变，电镜观察PI组和PS组海马细胞结构存在损伤；与PI组相比，PS组T1~T3时caspase?3表达减少（P<0.01），GAP?43和nNOS表达增加（P<0.01）。结论大鼠妊娠前吸入异氟烷或七氟烷对子代脑发育无不良影响，而妊娠期吸入异氟烷或七氟烷则可引起子代脑发育的短期异常，且异氟烷毒性作用大于七氟烷。
목적：탐토대서임신전후흡입이불완혹칠불완마취대자대대서뇌발육적영향。방법장30지자성SD대서수궤분위5조（매조6지）：대조조（C조）、임신전1 d흡입1.6%이불완6 h조（BI조）、임신후6、10、14화18 d시흡입1.6%이불완6 h조（PI조）、임신전1 d흡입2.4%칠불완6 h조（BS조）급임신후6、10、14화18 d흡입2.4%칠불완6 h조（PS조）。우출생당천（T1）、출생후제7（T2）、14（T3）화28천（T4）시각조안와별수궤취12지자서처사취해마，HE염색법관찰병이학개변，전경하관찰해마세포초미결구，면역조직화학염색법검측caspase?3、신경생장상관단백43（GAP?43）화신경형일양화담합매（nNOS）적표체。결과여C조상비，BI조화BS조각시점、PI조화PS조T4시점자대대서해마중caspase?3、GAP?43화nNOS적표체차이무통계학의의（P>0.05），HE염색급전경관찰해마조직미견차이。PI조화PS조T1~T3시caspase?3표체증가，GAP?43화nNOS표체감소（P<0.01）， PI조HE염색해마결구유교명현적병리개변，전경관찰PI조화PS조해마세포결구존재손상；여PI조상비，PS조T1~T3시caspase?3표체감소（P<0.01），GAP?43화nNOS표체증가（P<0.01）。결론대서임신전흡입이불완혹칠불완대자대뇌발육무불량영향，이임신기흡입이불완혹칠불완칙가인기자대뇌발육적단기이상，차이불완독성작용대우칠불완。
Objective To evaluate the effect of isoflurane or sevoflurane inhalation before and after gestation on the offspring brain development. Methods Thirty female adult Sprague Dawley rats were randomly assigned into 5 groups(n=6 each):control group(group C),group that ex?posed to isoflurane with the concentration of 1.6%for 6 hours before gestation(group BI),group that exposed to isoflurane with the concentration of 1.6%at the 6th,10th,14th and 18th day for 6 hours(group PI),group that exposed to sevoflurane with the concentration of 2.4%for 6 hours before gestation(group BS),and group exposed to sevoflurane with the concentration of 2.4%at the 6th,10th,14th and 18th day for 6 hours after gestation (group PS). Twelve offspring rats from pregnant rats in each group were chosen on the day of birth(T1),and 7th,14th and 28th days after birth(T2, T3 and T4)and sacrificed,and the hippocampi were then isolated. Hematoxylin and eosin stain were adopted to observe the tissue pathological change. Electron microscope was used to observe the neuron ultrastructure change of hippocampus. Immolunohistochemistry was used to detect cas?pase?3,the expression of growth associated protein?43(GAP?43)and neuronal nitric oxide synthase(nNOS). Results Compared with group C, no significant change was found in caspase?3,GAP?43 and nNOS expression in offspring rat hippocampus in groups BI and BS(P>0.05),and no damage change in hippocampal was seen by HE staining and electron microscopy. In group PI and PB,the expression of caspase?3 was significantly up?regulated,the expression of GAP?43 and nNOS was down?regulated at T1 to T3(P<0.01),and structural changes in cell were seen by electron microscopy. In group PI,significant pathological changes in hippocampal were seen by HE staining at T1 to T3. Compared with group PI,the expres?sion of GAP?43 and nNOS was significantly up?regulated(P<0.01),and the expression of caspase?3 was down?regulated at T1 to T3(P<0.01). Conclusion Isoflurane or sevoflurane inhalation before gestation does not affect the offspring brain development,while isoflurane or sevoflurane in?halation after gestation can induce transient abnormal change of offspring brain development,and isoflurane′s toxicity was greater than sevoflurane.