CAJ | 학술논문

目的：探讨增食欲素A（Orexin A）通过增食欲素受体1（OX1R）和AKT/PKB信号传导途径对胰岛细胞增殖的干预效应。方法体外培养的大鼠INS?1胰岛素瘤细胞暴露于不同浓度的Orexin A，OX1R拮抗剂（SB334867）、PI3K拮抗剂（渥曼青霉素）和AKT拮抗剂（PF?04691502）干预Orexin A的作用，测定INS?1的细胞增殖、凋亡、胰岛素分泌、OX1R蛋白活性及AKT蛋白磷酸化水平。结果 Orexin A（10-10~10-6 mol/L）可刺激INS?1细胞的增殖和活化，防止细胞凋亡，并增加胰岛素的分泌；Orexin A （10-10~10-6 mol/L）增强了INS?1细胞内AKT的磷酸化，SB334867（10-6 mol/L）、渥曼青霉素（10-8 mol/L）和PF?04691502（10-6 mol/L）可以减弱Orexin A的作用。结论 INS?1细胞内Orexin A通过Orexin A?OX1R的介导而活化AKT信号通路，促进细胞增殖。
목적：탐토증식욕소A（Orexin A）통과증식욕소수체1（OX1R）화AKT/PKB신호전도도경대이도세포증식적간예효응。방법체외배양적대서INS?1이도소류세포폭로우불동농도적Orexin A，OX1R길항제（SB334867）、PI3K길항제（악만청매소）화AKT길항제（PF?04691502）간예Orexin A적작용，측정INS?1적세포증식、조망、이도소분비、OX1R단백활성급AKT단백린산화수평。결과 Orexin A（10-10~10-6 mol/L）가자격INS?1세포적증식화활화，방지세포조망，병증가이도소적분비；Orexin A （10-10~10-6 mol/L）증강료INS?1세포내AKT적린산화，SB334867（10-6 mol/L）、악만청매소（10-8 mol/L）화PF?04691502（10-6 mol/L）가이감약Orexin A적작용。결론 INS?1세포내Orexin A통과Orexin A?OX1R적개도이활화AKT신호통로，촉진세포증식。
Objective To investigate the interference effects of orexin A on cell proliferation of the insulin?secreting beta?cell line(INS?1 cells) through the orexin receptor 1(OX1R)and the AKT/PKB signaling pathway. Methods INS?1 cells were exposed to different concentrations of orexin A in vitro,and treated with OX1R antagonist(SB334867),PI3K antagonist(wortmannin),or AKT antagonist(PF?04691502). The INS?1 cell proliferation and apoptosis,insulin secretion,OX1R protein activity and AKT phosphorylation level were determined. Results Orexin A(10-10 to 10-6 mol/L)stimulated the proliferation and activation of INS?1 cells,prevented apoptpsis,and increased insulin secretion. Additionally,AKT phosphorylation was stimulated by orexin A(10-10 to 10-6 mol/L). The OX1R antagonist SB334867(10-6 mol/L),the PI3K antagonist wortmannin (10-8 mol/L)and the AKT antagonist PF?04691502(10-6 mol/L)weakened the effects of orexin A. Conclusion Orexin A activated the AKT sig?naling pathway through the mediation of orexin A?OX1R,and promoted cell proliferation in INS?1 cells.