CAJ | 학술논문

目的：探讨姜黄素联合顺铂对非小细胞肺癌细胞A549放射增敏的影响。方法采用MTT法观察不同浓度姜黄素（10、20、50、100、200μmol／L）和顺铂（1、2、5、10、20 mg／L）作用24、48及72 h后的细胞存活率，根据实验设计分为单纯照射（ R）组、姜黄素＋照射（ C＋R）组、顺铂＋照射（ P＋R）组及姜黄素＋顺铂＋照射（ C＋P＋R）组，采用克隆形成实验检测4组经不同剂量X线（0、2、4、6、8、10 Gy）照射后的存活分数（SF）并通过单击多靶模型拟合细胞存活曲线计算增敏比（SER），分别采用人工划痕、Transwell试验及Western blotting检测以上4组处理24 h后的细胞迁移、侵袭及表皮生长因子受体（ EGFR）的蛋白水平。结果随姜黄素（10～200μmol／L范围内）和顺铂（1～20 mg／L范围内）浓度增加，A549细胞的细胞存活率降低，抑制效应呈浓度和时间依赖方式，差异有统计学意义（ P＜0?05）；C＋P＋R组在照射剂量为2～10 Gy时的细胞SF均低于其余3组，而C＋R组在4～10 Gy，P＋R组在2～10 Gy时的细胞SF均低于R组，差异有统计学意义（ P＜0?05），C＋R组、P＋R组及C＋P＋R组相对于R组的SER依次为1?24、1?31和1?96；C＋P＋R组的迁移距离、穿膜细胞数量及EGFR蛋白水平均低于其余3组，而C＋R组和P＋R组的以上指标亦低于R组，以上差异均有统计学意义（ P＜0?05）。结论姜黄素联合顺铂可抑制A549细胞增殖并具有放射增敏作用，同时抑制其迁移和侵袭，可能与EGFR相关信号通路受抑制有关。
목적：탐토강황소연합순박대비소세포폐암세포A549방사증민적영향。방법채용MTT법관찰불동농도강황소（10、20、50、100、200μmol／L）화순박（1、2、5、10、20 mg／L）작용24、48급72 h후적세포존활솔，근거실험설계분위단순조사（ R）조、강황소＋조사（ C＋R）조、순박＋조사（ P＋R）조급강황소＋순박＋조사（ C＋P＋R）조，채용극륭형성실험검측4조경불동제량X선（0、2、4、6、8、10 Gy）조사후적존활분수（SF）병통과단격다파모형의합세포존활곡선계산증민비（SER），분별채용인공화흔、Transwell시험급Western blotting검측이상4조처리24 h후적세포천이、침습급표피생장인자수체（ EGFR）적단백수평。결과수강황소（10～200μmol／L범위내）화순박（1～20 mg／L범위내）농도증가，A549세포적세포존활솔강저，억제효응정농도화시간의뢰방식，차이유통계학의의（ P＜0?05）；C＋P＋R조재조사제량위2～10 Gy시적세포SF균저우기여3조，이C＋R조재4～10 Gy，P＋R조재2～10 Gy시적세포SF균저우R조，차이유통계학의의（ P＜0?05），C＋R조、P＋R조급C＋P＋R조상대우R조적SER의차위1?24、1?31화1?96；C＋P＋R조적천이거리、천막세포수량급EGFR단백수평균저우기여3조，이C＋R조화P＋R조적이상지표역저우R조，이상차이균유통계학의의（ P＜0?05）。결론강황소연합순박가억제A549세포증식병구유방사증민작용，동시억제기천이화침습，가능여EGFR상관신호통로수억제유관。
Objective To explore the radiosensitization effect of curcumin plus cisplatin on non?small cell lung cancer A549 cells. Methods Cell viability at 24, 48 and 72 h after treatment with different concentrations ( 10, 20, 50, 100, 200 μmol/L) of curcumin or ( 1, 2, 5, 10, 20 mg/L) of cisplatin were determined by MTT. According to the experimental protocol, the below experi?ments were carried out in irradiation ( R ) group, curcumin+irradiation ( C+R ) group, cisplatin+irradiation ( P+R ) group and curcumin+cisplatin+irradiation ( C+P+R) group. The colony formation assay was employed to observe the surviving fraction ( SF) of a?bove four groups after X?ray irradiation of 0, 2, 4, 6, 8, 10 Gy. The artificial scratch, Transwell test and Western blotting were em?ployed to detect the cell migration, invasion and protein level of epidermal growth factor receptor ( EGFR) at 24 h after treatment in four groups. Results The cell viability of A549 cells gradually decreased with the increasing concentration of curcumin ranging from 10 to 200 μmol/L and cisplatin ranging from 1 to 20 mg/L in a dose?and time?dependent manner ( P<0?05) . The SF of C+P+R group were lower than the remaining 3 groups under the dose of 2?10 Gy ( P<0?05) . Compared with the R irradiation group, there was lower SF in C+R group under the dose of 4?10 Gy and P+R group under the dose of 2?10 Gy with significant difference ( P<0?05) . Compared with R group, the sensitizing enhancement ratio were 1?24, 1?31 and 1?96 in C+R group, P+R group and C+P+R group, respective?ly. There were lower migration distance, transmembrane cell number and EGFR protein level in C+P+R group versus the remaining 3 groups ( P<0?05) . Compared with the R group, the above indicators were also lower in C+R group and P+R group under the dose of 2?10 Gy with significant difference ( P<0?05) . Conclusion Curcumin plus cisplatin can inhibit the proliferation of A549 cells with radi?osensitizing effect and suppress its migration and invasion possibly via inhibition of EGFR signaling pathways.