临床肿瘤学杂志
臨床腫瘤學雜誌
림상종류학잡지
CHINESE CLINICAL ONCOLOGY
2015年
1期
79-82
,共4页
王璇%余波%马恒辉%周晓军%石群立%宋勇%王建东
王璇%餘波%馬恆輝%週曉軍%石群立%宋勇%王建東
왕선%여파%마항휘%주효군%석군립%송용%왕건동
非小细胞肺癌%Ventana免疫组织化学染色%EML4-ALK%基因重排
非小細胞肺癌%Ventana免疫組織化學染色%EML4-ALK%基因重排
비소세포폐암%Ventana면역조직화학염색%EML4-ALK%기인중배
Non-small cell lung cancer%Ventana immunohistochemical staining%EML4-ALK%Gene rearrangement
目的:分析Ventana免疫组织化学染色( IHC)检测非小细胞肺癌( NSCLC)组织中EML4?ALK融合基因的突变情况。解析Ventana IHC结果判读的难点和陷阱,为此项检测的开展提供参考。方法回顾性分析695份Ventana IHC检测NSCLC标本,对部分标本进行了实时定量PCR( qRT?PCR)对照研究。结果 EML4?ALK在腺癌中的突变率为8?78%,鳞状细胞癌中的突变率为4?49%,总突变率为8?48%。10例Ventana IHC为(-)和(+)标本qRT?PCR检测为阴性;5例Ventana IHC染色(+++)标本qRT?PCR检测均为阳性;5例Ventana IHC(++)标本qRT?PCR检测1例阳性。结论 EML4?ALK融合基因主要发生在肺腺癌。 Ventana IHC检测结果存在判读难点和陷阱,判读需要谨慎。 EML4?ALK IHC检测阳性(++)的需要qRT?PCR或其它方法进一步证实。
目的:分析Ventana免疫組織化學染色( IHC)檢測非小細胞肺癌( NSCLC)組織中EML4?ALK融閤基因的突變情況。解析Ventana IHC結果判讀的難點和陷阱,為此項檢測的開展提供參攷。方法迴顧性分析695份Ventana IHC檢測NSCLC標本,對部分標本進行瞭實時定量PCR( qRT?PCR)對照研究。結果 EML4?ALK在腺癌中的突變率為8?78%,鱗狀細胞癌中的突變率為4?49%,總突變率為8?48%。10例Ventana IHC為(-)和(+)標本qRT?PCR檢測為陰性;5例Ventana IHC染色(+++)標本qRT?PCR檢測均為暘性;5例Ventana IHC(++)標本qRT?PCR檢測1例暘性。結論 EML4?ALK融閤基因主要髮生在肺腺癌。 Ventana IHC檢測結果存在判讀難點和陷阱,判讀需要謹慎。 EML4?ALK IHC檢測暘性(++)的需要qRT?PCR或其它方法進一步證實。
목적:분석Ventana면역조직화학염색( IHC)검측비소세포폐암( NSCLC)조직중EML4?ALK융합기인적돌변정황。해석Ventana IHC결과판독적난점화함정,위차항검측적개전제공삼고。방법회고성분석695빈Ventana IHC검측NSCLC표본,대부분표본진행료실시정량PCR( qRT?PCR)대조연구。결과 EML4?ALK재선암중적돌변솔위8?78%,린상세포암중적돌변솔위4?49%,총돌변솔위8?48%。10례Ventana IHC위(-)화(+)표본qRT?PCR검측위음성;5례Ventana IHC염색(+++)표본qRT?PCR검측균위양성;5례Ventana IHC(++)표본qRT?PCR검측1례양성。결론 EML4?ALK융합기인주요발생재폐선암。 Ventana IHC검측결과존재판독난점화함정,판독수요근신。 EML4?ALK IHC검측양성(++)적수요qRT?PCR혹기타방법진일보증실。
Objective To analyze the occurrence of EML4?ALK gene rearrangement in non?small cell lung cancer ( NSCLC) detected by Ventana immunohistochemical staining (IHC), as to explore the challenges of Ventana IHC and provide a reference for other hospital to carry out the examination. Methods In this study, 659 cases of NSCLC were retrospectively analyzed and part of samples were checked by qRT?PCR. Results The occurrence rate of EML4?ALk in adenocarcinoma was 8?78% and in squamous cell carcinoma was 4?49%. Total occurrence in NSCLC was 8?48%. All cases with IHC staining (-) and (+) were confirmed negative mu?tation with qRT?PCR. Five cases with IHC staining (+++) were positive confirmed by qRT?PCR. One out of 5 cases with IHC staining (++) was confirmed positive by qRT?PCR. Conclusion EML4?ALK predominantly occurred in lung adenocarcinoma. There are some challenges in diagnosis of Ventana IHC. All cases with IHC staining (++) need to be verified by qRT?PCR or other methods.