CAJ | 학술논문

将瘦素（Leptin， LP）受体基因杂合型小鼠按4种方式进行配对，从子鼠鼠尾中提取基因组DNA，用PCR方法扩增LP受体基因片段，琼脂糖凝胶电泳后观察。结果表明， LP+/+、 LP+/-、 LP-/-各表型小鼠互交繁殖结果基本符合孟德尔遗传规律，且雌、雄性LP-/-小鼠（即DB/DB小鼠）无繁殖能力。雌、雄性LP+/-小鼠交配能高效地繁殖LP-/-小鼠； PCR方法能够精确地鉴定LP-/-小鼠。
장수소（Leptin， LP）수체기인잡합형소서안4충방식진행배대，종자서서미중제취기인조DNA，용PCR방법확증LP수체기인편단，경지당응효전영후관찰。결과표명， LP+/+、 LP+/-、 LP-/-각표형소서호교번식결과기본부합맹덕이유전규률，차자、웅성LP-/-소서（즉DB/DB소서）무번식능력。자、웅성LP+/-소서교배능고효지번식LP-/-소서； PCR방법능구정학지감정LP-/-소서。
The leptin receptor gene heterozygous mice were matedin four ways. Genomic DNA was isolated from tails and analyzed by PCR . The result showed that the ratio of the offspring genotypes fit the Mendel’s laws . The male and female LP -/-mice were infertile . It was efficient to breed leptin receptor gene knockout homozygote mice by inbreeding of the heterozygotes . PCR methods could be used to identify the genotype of the LP -/-mice precisely .