CAJ | 학술논문

目的：探讨TMTP1-DKK在治疗高转移宫颈癌的潜在应用价值。方法选择高转移和低转移配对宫颈癌细胞系SiHa和C-33A,Transwell试验分别确证其转移能力；Rhodamine标记 TMTP1,免疫荧光检测Rhodamine-TMTP1对SiHa、C-33A的亲和能力；CCK8检测TMTP1-DKK和TMTP1-VK处理后对SiHa和C-33A细胞的杀伤率,Transewell检测TMTP1-DKK和TMTP1-VK处理后对SiHa细胞侵袭能力的影响。结果 SiHa细胞侵袭能力强于C-33A,TMTP1亲和实验显示 TMTP1对高转移细胞系 SiHa 亲和力明显高于 C-33A,TMTP1-DKK对SiHa生存率影响明显强于C-33A,差异有统计学意义(P<0．05)。结论 TMTP1-DKK能特异性地杀伤高侵袭能力的宫颈癌细胞SiHa,但对低转移能力细胞C-33A杀伤效应不明显。提示TMTP1-DKK可以作为高转移宫颈癌靶向化疗药物的可能性。
목적：탐토TMTP1-DKK재치료고전이궁경암적잠재응용개치。방법선택고전이화저전이배대궁경암세포계SiHa화C-33A,Transwell시험분별학증기전이능력；Rhodamine표기 TMTP1,면역형광검측Rhodamine-TMTP1대SiHa、C-33A적친화능력；CCK8검측TMTP1-DKK화TMTP1-VK처리후대SiHa화C-33A세포적살상솔,Transewell검측TMTP1-DKK화TMTP1-VK처리후대SiHa세포침습능력적영향。결과 SiHa세포침습능력강우C-33A,TMTP1친화실험현시 TMTP1대고전이세포계 SiHa 친화력명현고우 C-33A,TMTP1-DKK대SiHa생존솔영향명현강우C-33A,차이유통계학의의(P<0．05)。결론 TMTP1-DKK능특이성지살상고침습능력적궁경암세포SiHa,단대저전이능력세포C-33A살상효응불명현。제시TMTP1-DKK가이작위고전이궁경암파향화료약물적가능성。
Objective To investigate the remarkable specificity and anti-tumor ability of peptide TMTP1-DKK on highly metastatic cervical tumors in vitro. Methods Cervical cancer cells SiHa and C-33A that had different meta-static capacities were used. The cell invasion was investigated using the Transwell system. Rhodamine was coupled to the peptides TMTP1. Immunofluorescence assay was carried out to determine the specific binding capacities of the TMTP1,SiHa and C-33A. The inhibition rates of cells growth was measured by CKK-8 assay. The SiHa and C-33A cells were treated with different concentrations of TMTP1-DKK and TMTP1-VK in vitro,and the biological behavior changes of cells were assessed by migration and invasion through Transwell assay. Results Cervical cancer cell SiHa had higher metastatic ability than C-33A. Rhodamine-TMTP1 bound specifically to highly metastatic tumor cell line,Si-Ha,however,Rhodamine-TMTP1 did not bind to thenometastatic cervical cancer cell C-33A. TMTP1-DKK showed higher anti-tumor effects on highly metastatic SiHa compared to C33A,and the difference was significant(P<0. 05). Conclusion TMTP1-DKK can inhibit the proliferation of highly metastatic cervical cancer cell SiHa,but it has little effect onnometastatic cervical cancer cell C-33A. These results suggest TMTP1-DKK may be a powerful candidate ther-apeutic agent for metastatic tumors.