江西农业大学学报
江西農業大學學報
강서농업대학학보
ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSIS
2015年
1期
135-140
,共6页
刘姚%傅凌韵%李楠%王文君
劉姚%傅凌韻%李楠%王文君
류요%부릉운%리남%왕문군
青钱柳低聚糖%重均分子量%3T3-L1脂肪细胞%PPARγ%C/EBPα
青錢柳低聚糖%重均分子量%3T3-L1脂肪細胞%PPARγ%C/EBPα
청전류저취당%중균분자량%3T3-L1지방세포%PPARγ%C/EBPα
Cyclocarya paliurus oligosaccharide%weight-average molecular weight%3T3-L1 adipocyte%PPARγ%C/EBPα
探讨青钱柳低聚糖（ Cyclocarya paliurus oligosaccharide，CPO）对3T3－L1脂肪细胞增殖分化及相关基因表达的影响。采用水提醇的方法得到青钱柳初多糖，经D301－R大孔吸附树脂和DEAE纤维素阴离子交换树脂分离纯化，收集CPO作用于3T3－L1前脂肪细胞，并采用Q－PCR测定相关基因过氧化物酶体增殖物激活受体γ（ peroxisomes proliferator－activated receptor γ， PPARγ）和 CAAT／增强子结合蛋白α（ CAAT／enhancer binding proteinα，C／EBPα） mRNA表达。结果表明：CPO含有2个组分，其重均分子量分别为3842 Da和1481 Da；CPO在低浓度时能促进脂肪细胞的增殖，高浓度时能抑制脂肪细胞的增殖；对脂肪细胞分化的影响较小。 CPO可抑制PPARγ和C／EBPα mRNA表达，与对照组比较差异有极显著性意义（P＜0．01）。表明高浓度的CPO抑制脂肪细胞增殖分化水平，其机制可能与抑制PPARγ和C／EBPα mRNA表达有关。
探討青錢柳低聚糖（ Cyclocarya paliurus oligosaccharide，CPO）對3T3－L1脂肪細胞增殖分化及相關基因錶達的影響。採用水提醇的方法得到青錢柳初多糖，經D301－R大孔吸附樹脂和DEAE纖維素陰離子交換樹脂分離純化，收集CPO作用于3T3－L1前脂肪細胞，併採用Q－PCR測定相關基因過氧化物酶體增殖物激活受體γ（ peroxisomes proliferator－activated receptor γ， PPARγ）和 CAAT／增彊子結閤蛋白α（ CAAT／enhancer binding proteinα，C／EBPα） mRNA錶達。結果錶明：CPO含有2箇組分，其重均分子量分彆為3842 Da和1481 Da；CPO在低濃度時能促進脂肪細胞的增殖，高濃度時能抑製脂肪細胞的增殖；對脂肪細胞分化的影響較小。 CPO可抑製PPARγ和C／EBPα mRNA錶達，與對照組比較差異有極顯著性意義（P＜0．01）。錶明高濃度的CPO抑製脂肪細胞增殖分化水平，其機製可能與抑製PPARγ和C／EBPα mRNA錶達有關。
탐토청전류저취당（ Cyclocarya paliurus oligosaccharide，CPO）대3T3－L1지방세포증식분화급상관기인표체적영향。채용수제순적방법득도청전류초다당，경D301－R대공흡부수지화DEAE섬유소음리자교환수지분리순화，수집CPO작용우3T3－L1전지방세포，병채용Q－PCR측정상관기인과양화물매체증식물격활수체γ（ peroxisomes proliferator－activated receptor γ， PPARγ）화 CAAT／증강자결합단백α（ CAAT／enhancer binding proteinα，C／EBPα） mRNA표체。결과표명：CPO함유2개조분，기중균분자량분별위3842 Da화1481 Da；CPO재저농도시능촉진지방세포적증식，고농도시능억제지방세포적증식；대지방세포분화적영향교소。 CPO가억제PPARγ화C／EBPα mRNA표체，여대조조비교차이유겁현저성의의（P＜0．01）。표명고농도적CPO억제지방세포증식분화수평，기궤제가능여억제PPARγ화C／EBPα mRNA표체유관。
The effect of Cyclocarya paliurus oligosaccharide ( CPO) on the proliferation and differentiation of 3T3?L1 adipocytes and the related gene mRNA expression was investigated.CPO was obtained through water solution and alcohol sedimentation,and isolated and purified with D301?R macroporous resins and DEAE cel?lulose anion exchange resins.The molecular weight was measured with the GPC and MALLS methods.The effect of CPO on the proliferation and differentiation of 3T3?L1 adipocytes was studied,and the related expression of PPARγand C/EBPαmRNA were determined by Q?PCR.The results showed that CPO consisted of two differ?ent components and their weight?average molecular weights (Mp) were 3 842 Da and 1 481 Da.CPO promoted the proliferation at the low concentration and restrained it at the high concentration.CPO decreased the expres?sion of PPARγand C/EBPαwith significant differences from the control group(P<0.01).The results suggested that hith?dosaged CPO restrained the proliferation and differentiation of 3T3?L1 adipocytes,which might be re?lated with its inhibiting the expression of PPARγ and C/EBPα.
