CAJ | 학술논문

目的：观察氟对体外培养的大鼠成釉细胞 HAT-7细胞活性及细胞内 Ca2＋浓度的影响。方法：取对数生长期的 HAT-7细胞，分别加入不同浓度的 NaF 培养液，培养24、48、72 h 后，用 CCK-8检测细胞的活性；流式细胞术分析氟对细胞凋亡的影响；激光共聚焦显微镜检测细胞内钙离子的浓度。结果：NaF 浓度为0．4、0．8 mmol／L 时，促进成釉细胞增殖；当 NaF 浓度大于1．6 mmol／L 时，促进成釉细胞凋亡，NaF 浓度为1．6 mmol／L 时，细胞早期凋亡数量增加，激光共聚焦显微镜检测证实，1．6 mmol／L NaF 可以诱导大鼠成釉细胞内 Ca2＋浓度升高，NaF 对 HAT-7细胞的上述作用与浓度和作用时间呈正相关。结论：低浓度氟促进 HAT-7细胞增殖，高浓度则抑制其增殖。NaF 浓度超过1．6 mmol／L 时，可诱导成釉细胞凋亡，并诱导成釉细胞内Ca2＋浓度增加。
목적：관찰불대체외배양적대서성유세포 HAT-7세포활성급세포내 Ca2＋농도적영향。방법：취대수생장기적 HAT-7세포，분별가입불동농도적 NaF 배양액，배양24、48、72 h 후，용 CCK-8검측세포적활성；류식세포술분석불대세포조망적영향；격광공취초현미경검측세포내개리자적농도。결과：NaF 농도위0．4、0．8 mmol／L 시，촉진성유세포증식；당 NaF 농도대우1．6 mmol／L 시，촉진성유세포조망，NaF 농도위1．6 mmol／L 시，세포조기조망수량증가，격광공취초현미경검측증실，1．6 mmol／L NaF 가이유도대서성유세포내 Ca2＋농도승고，NaF 대 HAT-7세포적상술작용여농도화작용시간정정상관。결론：저농도불촉진 HAT-7세포증식，고농도칙억제기증식。NaF 농도초과1．6 mmol／L 시，가유도성유세포조망，병유도성유세포내Ca2＋농도증가。
Objective:To evaluate the effect of fluoride on the viability of rat ameloblast HAT-7 cells and calcium concentration in the cells.Methods:HAT-7 cells were exposed to NaF at 0,0.4,0.8,1.6,3.2 and 6.4 mmol/L for 24,48 and 72 h respectively. CCK-8 assay was performed to examine the cells proliferation;the apoptosis rate was determined by flow cytometry;Ca2 +concentration in the cells was detected by laser scanning confocal microscopy.Results:The cell proliferation was increased by NaF at 0.4 mmol/L and 0.8 mmol/L,whereas inhibited at 1.6 mmol/L and above.The effects were in a time-dependent manner.NaF increased apoptosis of the cells and increased Ca2 + concentration in the cells in a concentration-dependent manner.Conclusion:Fluoride at low doses promotes proliferation,at high doses inhibits proliferation of HAT-7 cells.NaF of 1.6 mmol/L or more induces apoptosis of HAT-7 cells and in-duce Ca2 + overloading in the cells.