CAJ | 학술논문

目的：检测Wnt信号通路的关键组分β-catenin蛋白在管腔A、管腔B、HER-2过表达、基底细胞样及未分类型(基底细胞样及未分类型统称为三阴型)乳腺癌中的表达及细胞内定位，及其与乳腺癌临床病理参数的关系。方法采用免疫组化EliVison两步法检测58例乳腺癌中β-catenin蛋白表达及细胞内定位。结果(1)管腔A、管腔B、HER-2过表达、基底细胞样及未分类型乳腺癌中β-catenin胞质异常表达阳性率分别为21．1%、50%、60%、100%及60%(三阴型84．6%)，差异有显著性(P<0．01)。β-catenin高胞质表达与基底细胞样及三阴型乳腺癌相关(P<0．05)。(2)β-catenin胞质异常表达与组织学分级相关(P<0．01)，与淋巴结转移、肿瘤大小、患者年龄无关；与 E-cadherin 膜表达缺失、Ki-67及 CK5/6表达呈正相关(P <0．05)，与ER及PR表达呈负相关(P<0．01)。结论乳腺癌中Wnt信号通路的异常与组织学分级、Ki-67及CK5/6表达呈正相关，与ER、PR表达呈负相关。基底细胞样及三阴型分子亚型有更高频率的Wnt信号异常。β-catenin有可能成为基底细胞样及三阴型乳腺癌的治疗靶点。
목적：검측Wnt신호통로적관건조분β-catenin단백재관강A、관강B、HER-2과표체、기저세포양급미분류형(기저세포양급미분류형통칭위삼음형)유선암중적표체급세포내정위，급기여유선암림상병리삼수적관계。방법채용면역조화EliVison량보법검측58례유선암중β-catenin단백표체급세포내정위。결과(1)관강A、관강B、HER-2과표체、기저세포양급미분류형유선암중β-catenin포질이상표체양성솔분별위21．1%、50%、60%、100%급60%(삼음형84．6%)，차이유현저성(P<0．01)。β-catenin고포질표체여기저세포양급삼음형유선암상관(P<0．05)。(2)β-catenin포질이상표체여조직학분급상관(P<0．01)，여림파결전이、종류대소、환자년령무관；여 E-cadherin 막표체결실、Ki-67급 CK5/6표체정정상관(P <0．05)，여ER급PR표체정부상관(P<0．01)。결론유선암중Wnt신호통로적이상여조직학분급、Ki-67급CK5/6표체정정상관，여ER、PR표체정부상관。기저세포양급삼음형분자아형유경고빈솔적Wnt신호이상。β-catenin유가능성위기저세포양급삼음형유선암적치료파점。
Purpose To study the levels and subcellular localization of β-catenin in 5 different molecular subtypes of breast carcino-mas. Methods An immunohistochemical study was undertaken for measuring the levels and subcellular localization ofβ-catenin in 58 breast carcinomas. Results ( 1 ) The cytoplasmic expression of β-catenin was 21. 1%, 50%, 60%, 100% and 60% ( TNBC 84. 6%) in Lumina A, Lumina B, HER-2-OE, basal-like breast carcinoma ( BLBC) and uncl phenotype respectively. High cytoplas-mic expression was associated with the BLBC and TNBC subtypes ( P<0. 05 ) . ( 2 ) An association was identified between high cyto-plasmic expression of β-catenin, and high tumor grade (P<0. 01), abnormal E-cadherin, positive Ki-67 and CK5/6 (P<0. 05), negative ER and PR (P<0. 01), but no association was observed for lymph node metastasis, tumor size and patients’age. Conclu-sion An association is identified between high cytoplasmic expression, and high tumor grade, positive Ki-67 and CK5/6, negative ER and PR, that means a high cytoplasmic expression ofβ-catenin is associated with an adverse outcome in breast cancer. High cytoplas-mic expression are associated with the BLBC and TNBC subtypes whcih recognizing Wnt signaling as a rational target in TNBC and BLBC. The results of the study have implications for therapeutic target identification and the design of future clinical trials for TNBC and BLBC.