CAJ | 학술논문

目的：研究姜黄素体外对人结肠癌Lovo细胞血管内皮生长因子VEGF表达的影响。方法不同浓度(0μmol/L、5μmol/L、10μmol/L、20μmol/L、40μmol/L)姜黄素处理2013年8月—2014年2月中南大学提供的人结肠癌细胞株Lovo24 h后，用Real-time PCR检测姜黄素对人结肠癌细胞株血管内皮生长因子VEGF mRNA表达的变化，用Western blot法检测姜黄素对人结肠癌细胞株VEGF蛋白表达的变化。结果人结肠癌Lovo细胞经过姜黄素处理24 h后，5μmol/L、10μmol/L、20μmol/L、40μmol/L姜黄素组VEGF mRNA及蛋白的表达均明显下降，与对照组比较差异有统计学意义（P<0.05)。结论姜黄素能下调人结肠癌Lovo细胞VEGF的表达，并呈剂量依赖，这可能是姜黄素抑制结肠癌Lovo细胞增殖及侵袭性的机制之一。
목적：연구강황소체외대인결장암Lovo세포혈관내피생장인자VEGF표체적영향。방법불동농도(0μmol/L、5μmol/L、10μmol/L、20μmol/L、40μmol/L)강황소처리2013년8월—2014년2월중남대학제공적인결장암세포주Lovo24 h후，용Real-time PCR검측강황소대인결장암세포주혈관내피생장인자VEGF mRNA표체적변화，용Western blot법검측강황소대인결장암세포주VEGF단백표체적변화。결과인결장암Lovo세포경과강황소처리24 h후，5μmol/L、10μmol/L、20μmol/L、40μmol/L강황소조VEGF mRNA급단백적표체균명현하강，여대조조비교차이유통계학의의（P<0.05)。결론강황소능하조인결장암Lovo세포VEGF적표체，병정제량의뢰，저가능시강황소억제결장암Lovo세포증식급침습성적궤제지일。
Objective To study the effect of curcumin on the expression of VEGF in human colon cancer Lovo cells in vitro. Methods The human colon cancer cell lines Lovo were treated with 0 μmol/L,5 μmol/L10 μmol/L,20 μmol/L, 40 μmol/L curcum-in for 24 hours respectively, then we detected the human colon cancer cell lines VEGF mRNA expression by Real-time PCR and VEGF protein expression by Western Blot. Results After the human colon cancer cell lines Lovo cells treated with curcumin for 24 hours respectively, the expression of VEGF mRNA and protein of the curcumin-treated groups (5 μmol/L,10 μmol/L,20 μmol/L, 40 μmol/L) had more significant reduction compared with control group (P<0.05). Conclusion Curcumin could decrease the ex-pression of VEGF in human colon cancer Lovo cells dose-dependently, this may provide some evidence for the mechanism that curcumin could inhibit the proliferation and invasion of colon cancer Lovo cells.