CAJ | 학술논문

目的：探讨妊娠合并HBV感染的孕妇母婴垂直传播的相关影响因素。方法收集100例乙肝病毒携带孕妇及其100例新生儿作为研究对象,采用双抗体夹心时间分辨免疫荧光法(IFMA法)检测孕妇HBsAg和HBeAg,实时荧光定量PCR法检测HBV DNA载量,并用流式细胞仪检测新生儿脐血中NK细胞CD69和IFN-γ,并对以上指标的相关性进行统计分析。结果母血中HBsAg单阳性与HBsAg/HBeAg双阳性组间母婴垂直传播比率差异有统计学意义(P<0.001)》孕妇外周血HBV DNA载量越高母婴垂直传播几率越高。妊娠合并乙型肝炎患者中HBV母婴垂直传播组﹑未发生HBV母婴垂直传播患者与正常对照组三组间脐血中T淋巴细胞中活化标志物CD69表达的细胞百分比分别为：(9.58±1.745)%﹑(15.6±2.117)%和(5.66±3.724)%,差异有统计学意义(P<0.001)。 HBV母婴垂直传播与IFN-γ无明显相关性(P>0.05)。结论母血HBeAg阳性与否﹑HBV DNA病毒载量高低直接决定着母婴HBV垂直传递的风险,同时T淋巴细胞中活化标志物CD69表达水平与母婴HBV垂直传播相关,该研究的结果为临床研究HBV母婴传播的机理及其预防措施,减少HBV携带者,提高新生儿出生质量有重要意义。
목적：탐토임신합병HBV감염적잉부모영수직전파적상관영향인소。방법수집100례을간병독휴대잉부급기100례신생인작위연구대상,채용쌍항체협심시간분변면역형광법(IFMA법)검측잉부HBsAg화HBeAg,실시형광정량PCR법검측HBV DNA재량,병용류식세포의검측신생인제혈중NK세포CD69화IFN-γ,병대이상지표적상관성진행통계분석。결과모혈중HBsAg단양성여HBsAg/HBeAg쌍양성조간모영수직전파비솔차이유통계학의의(P<0.001)》잉부외주혈HBV DNA재량월고모영수직전파궤솔월고。임신합병을형간염환자중HBV모영수직전파조﹑미발생HBV모영수직전파환자여정상대조조삼조간제혈중T림파세포중활화표지물CD69표체적세포백분비분별위：(9.58±1.745)%﹑(15.6±2.117)%화(5.66±3.724)%,차이유통계학의의(P<0.001)。 HBV모영수직전파여IFN-γ무명현상관성(P>0.05)。결론모혈HBeAg양성여부﹑HBV DNA병독재량고저직접결정착모영HBV수직전체적풍험,동시T림파세포중활화표지물CD69표체수평여모영HBV수직전파상관,해연구적결과위림상연구HBV모영전파적궤리급기예방조시,감소HBV휴대자,제고신생인출생질량유중요의의。
Objective To investigate the influencing factors related to mother-to-child vertical transmission of hepatitis B virus infection. Methods 100 cases of hepatitis B virus carrying pregnant women and their 100 newborns were selected as the research object. HBsAg and HBeAg of the pregnant women were detected by IFMA method, and HBV DNA load of them was detected by real-time PCR. NK cells CD69 and IFN-γ in the umbilical cord blood of the newborns were detected by flow cytometry. And the relativity between the above indexes was analyzed statistically. Results The difference in the ratio of mother-to-child vertical transmission of HBV between the pregnant women with positive HBsAg and those with double positive HBsAg/HBeAg was statisti-cally significant(P<0.001). The higher HBV DNA load in the maternal blood, the higher HBV mother-to-child vertical transmission ratio would be. T lymphocyte activation marker CD69 expression levels in the hepatitis group with mother-to-child vertical trans-mission of HBV, hepatitis group without mother-to-child vertical transmission of HBV, and the normal control group was (9.58± 1.745)%, (15.6±2.117)%, (5.66±3.724)%, respectively, the difference was statistically significant(P<0.001). And mother-to-child vertical transmission of HBV had no significant relativity with IFN-γ(P>0.05). Conclusion Positive HBeAg and HBV DNA load in the maternal blood are associated with the risk of mother-to-child vertical transmission of HBV. And T lymphocyte activation marker CD69 expression levels are related to mother-to-child vertical transmission of HBV. The results of this study are about the mechanism and preventive measures of mother-to-child vertical transmission of HBV clinically, which are of great significance in reducing the HBV carriers and improving the quality of neonates.