CAJ | 학술논문

目的：探究不同病理分级的宫颈鳞状细胞癌患者血清及癌组织中T细胞免疫球蛋白黏蛋白3(Tim-3)及其配体半乳凝素9(Gal-9)表达,分析其临床意义。方法收集17例上皮瘤样病变(CIN),38例含低(PSCC)﹑中(MSCC)﹑高分化(WSCC)宫颈鳞状细胞癌(SCC)组织以及血清样本,17例正常妇女血清,并依次分为CIN组﹑PSCC组﹑MSCC组﹑WSCC组以及正常组。分别采用蛋白质免疫杂交技术(Western blot)以及qRT-PCR技术检测CIN组﹑PSCC组﹑MSCC组以及WSCC组组织样本中Tim-3以及Gal-9的表达,采用酶联免疫吸附法(ELISA)检测CIN组﹑PSCC组﹑MSCC组﹑WSCC组以及正常组血清样本中Gal-9的表达水平。分别比较各组组织中Tim-3和Gal-9的表达以及血清中Gal-9的表达水平与宫颈鳞状细胞癌不同病理分级的相关性。结果 Western blot与qRT-PCR结果均显示Tim-3和Gal-9在SCC各组组织中表达高于CIN组(P<0.05),且与病理分级呈正相关(r=0.738﹑0.671,P<0.05)。 ELISA结果亦显示Gal-9在SCC各组血清中高于正常组以及CIN组(P<0.05),切与病理分级呈正相关性(r=0.687,P<0.05)。结论 Tim-3及其配体Gal-9的表达与宫颈鳞状细胞癌病理分级成正相关,Tim-3以及Gal-9可作为临床诊断及预后的有义指标。
목적：탐구불동병리분급적궁경린상세포암환자혈청급암조직중T세포면역구단백점단백3(Tim-3)급기배체반유응소9(Gal-9)표체,분석기림상의의。방법수집17례상피류양병변(CIN),38례함저(PSCC)﹑중(MSCC)﹑고분화(WSCC)궁경린상세포암(SCC)조직이급혈청양본,17례정상부녀혈청,병의차분위CIN조﹑PSCC조﹑MSCC조﹑WSCC조이급정상조。분별채용단백질면역잡교기술(Western blot)이급qRT-PCR기술검측CIN조﹑PSCC조﹑MSCC조이급WSCC조조직양본중Tim-3이급Gal-9적표체,채용매련면역흡부법(ELISA)검측CIN조﹑PSCC조﹑MSCC조﹑WSCC조이급정상조혈청양본중Gal-9적표체수평。분별비교각조조직중Tim-3화Gal-9적표체이급혈청중Gal-9적표체수평여궁경린상세포암불동병리분급적상관성。결과 Western blot여qRT-PCR결과균현시Tim-3화Gal-9재SCC각조조직중표체고우CIN조(P<0.05),차여병리분급정정상관(r=0.738﹑0.671,P<0.05)。 ELISA결과역현시Gal-9재SCC각조혈청중고우정상조이급CIN조(P<0.05),절여병리분급정정상관성(r=0.687,P<0.05)。결론 Tim-3급기배체Gal-9적표체여궁경린상세포암병리분급성정상관,Tim-3이급Gal-9가작위림상진단급예후적유의지표。
Objective Our study investigates the expression of T cell immunoglobulin mucin 3 (Tim-3) and its ligand galectin-9 (Gal-9) in the serum and cancer tissue in patients with squamous cell carcinoma of the cervix of different pathological gradings and analyzes its clinical significance. Methods We collected the samples of tissues and serum of 17 cases of cervical intraepithe-lial neoplasia (CIN), 38 cases of squamous cell carcinoma(SCC) of the cervix including poorly differentiated squamous cell carcino-ma(PSCC) of the cervix, moderately differentiated squamous cell carcinoma (MSCC) of the cervix, well differentiated squamous cell carcinoma (WSCC) of the cervix, and 17 cases of normal women and grouped them as the CIN group, PSCC group, MSCC group, WSCC group, normal group in turn. The expressions of Tim-3 and Gal-9 in the tissues of CIN, PSCC, MSCC, WSCC group de-tected by Western blot and qRT-PCR were compared between the groups and the expressions of Gal-9 in the serum of the above groups and the normal group detected by ELISA were also compared between the groups so as to find the correlation between their expressions and pathological grading of squamous cell carcinoma of the cervix. Results The result of Western blot and qRT-PCR showed that Tim-3 and Gal-9 expression in the tissues of SCC groups were higher than that in the CIN group(P<0.05), and Tim-3 and Gal-9 had positive correlation with pathological gradings (r=0.738, 0.671, P<0.05). The result of ELISA showed that the ex-pression of Gal-9 in the serum of SCC groups was higher than that in the normal group and CIN group (P<0.05), and Gal-9 had positive correlation with pathological gradings (r=0.687, P<0.05). Conclusion The expression of Tim-3 and its ligand Gal-9 has positive correlation with the pathological gradings of squamous cell carcinoma of the cervix. Tim-3 and Gal-9 can be the signifi-cant indicators of clinical diagnosis and prognosis.