CAJ | 학술논문

目的：观察补肾养血明目方对实验性视网膜变性的干预作用，为临床应用提供客观依据。方法将碘酸钠腹腔注射诱导的视网膜变性C57BL/6N小鼠按随机数字表法随机分为3组：蒸馏水组，补肾养血明目方组和正常对照组，前两组自造模后第1天分别用蒸馏水、补肾养血明目方煎剂灌胃治疗，正常组小鼠不予造模，常规饲养。分别于治疗后7 d、14 d时采用视网膜电生理检查技术、病理学及TUNEL方法观察各组视网膜的生理功能和病变程度。结果治疗后7 d，3组小鼠的暗适应视网膜电图（d-ERG）、振荡电位（OPs）和明适应视网膜电图（l-ERG）的振幅相比较，差异有统计学意义（P＜0.05）。病理切片显示蒸馏水组及补肾养血明目方组的视网膜色素上皮（RPE）细胞数量均减少，外核层出现排列紊乱和波浪状改变，但补肾养血明目方组的病变程度较蒸馏水组轻，两组病变范围得分分别为3.18±0.603和3.64±0.924，组间差异无统计学意义（P＞0.05）。 TUNEL法检测补肾养血明目方组和蒸馏水组RPE细胞的凋亡率分别为36.2%和60.7%，差异有统计学意义（P＜0.05）。治疗后14 d，3组小鼠视网膜电图d-ERG、OPs、l-ERG间的振幅差异有统计学意义（P＜0.05）。 HE染色结果显示2组造模小鼠的视网膜外核层细胞数目也减少，但病变的程度和范围较治疗后7 d时轻，补肾养血明目方组和蒸馏水组病变范围得分分别为2.09±0.539和2.73±0.647，2组相比差异有统计学意义（P＜0.05）。 TUNEL检测显示补肾养血明目方组RPE凋亡细胞数少于蒸馏水组，且外核层未见细胞凋亡现象，补肾养血明目方组和蒸馏水组凋亡率分别为28.8%和51.5%，差异有统计学意义（P＜0.05）。结论补肾养血明目方对实验性视网膜变性有干预作用，可以减轻视网膜病变程度和减少视网膜色素上皮细胞和感光细胞的凋亡，其作用机制有待进一步探讨。目的：觀察補腎養血明目方對實驗性視網膜變性的榦預作用，為臨床應用提供客觀依據。方法將碘痠鈉腹腔註射誘導的視網膜變性C57BL/6N小鼠按隨機數字錶法隨機分為3組：蒸餾水組，補腎養血明目方組和正常對照組，前兩組自造模後第1天分彆用蒸餾水、補腎養血明目方煎劑灌胃治療，正常組小鼠不予造模，常規飼養。分彆于治療後7 d、14 d時採用視網膜電生理檢查技術、病理學及TUNEL方法觀察各組視網膜的生理功能和病變程度。結果治療後7 d，3組小鼠的暗適應視網膜電圖（d-ERG）、振盪電位（OPs）和明適應視網膜電圖（l-ERG）的振幅相比較，差異有統計學意義（P＜0.05）。病理切片顯示蒸餾水組及補腎養血明目方組的視網膜色素上皮（RPE）細胞數量均減少，外覈層齣現排列紊亂和波浪狀改變，但補腎養血明目方組的病變程度較蒸餾水組輕，兩組病變範圍得分分彆為3.18±0.603和3.64±0.924，組間差異無統計學意義（P＞0.05）。 TUNEL法檢測補腎養血明目方組和蒸餾水組RPE細胞的凋亡率分彆為36.2%和60.7%，差異有統計學意義（P＜0.05）。治療後14 d，3組小鼠視網膜電圖d-ERG、OPs、l-ERG間的振幅差異有統計學意義（P＜0.05）。 HE染色結果顯示2組造模小鼠的視網膜外覈層細胞數目也減少，但病變的程度和範圍較治療後7 d時輕，補腎養血明目方組和蒸餾水組病變範圍得分分彆為2.09±0.539和2.73±0.647，2組相比差異有統計學意義（P＜0.05）。 TUNEL檢測顯示補腎養血明目方組RPE凋亡細胞數少于蒸餾水組，且外覈層未見細胞凋亡現象，補腎養血明目方組和蒸餾水組凋亡率分彆為28.8%和51.5%，差異有統計學意義（P＜0.05）。結論補腎養血明目方對實驗性視網膜變性有榦預作用，可以減輕視網膜病變程度和減少視網膜色素上皮細胞和感光細胞的凋亡，其作用機製有待進一步探討。
목적：관찰보신양혈명목방대실험성시망막변성적간예작용，위림상응용제공객관의거。방법장전산납복강주사유도적시망막변성C57BL/6N소서안수궤수자표법수궤분위3조：증류수조，보신양혈명목방조화정상대조조，전량조자조모후제1천분별용증류수、보신양혈명목방전제관위치료，정상조소서불여조모，상규사양。분별우치료후7 d、14 d시채용시망막전생리검사기술、병이학급TUNEL방법관찰각조시망막적생리공능화병변정도。결과치료후7 d，3조소서적암괄응시망막전도（d-ERG）、진탕전위（OPs）화명괄응시망막전도（l-ERG）적진폭상비교，차이유통계학의의（P＜0.05）。병리절편현시증류수조급보신양혈명목방조적시망막색소상피（RPE）세포수량균감소，외핵층출현배렬문란화파랑상개변，단보신양혈명목방조적병변정도교증류수조경，량조병변범위득분분별위3.18±0.603화3.64±0.924，조간차이무통계학의의（P＞0.05）。 TUNEL법검측보신양혈명목방조화증류수조RPE세포적조망솔분별위36.2%화60.7%，차이유통계학의의（P＜0.05）。치료후14 d，3조소서시망막전도d-ERG、OPs、l-ERG간적진폭차이유통계학의의（P＜0.05）。 HE염색결과현시2조조모소서적시망막외핵층세포수목야감소，단병변적정도화범위교치료후7 d시경，보신양혈명목방조화증류수조병변범위득분분별위2.09±0.539화2.73±0.647，2조상비차이유통계학의의（P＜0.05）。 TUNEL검측현시보신양혈명목방조RPE조망세포수소우증류수조，차외핵층미견세포조망현상，보신양혈명목방조화증류수조조망솔분별위28.8%화51.5%，차이유통계학의의（P＜0.05）。결론보신양혈명목방대실험성시망막변성유간예작용，가이감경시망막병변정도화감소시망막색소상피세포화감광세포적조망，기작용궤제유대진일보탐토。
OBJECTIVE To observe the intervention effect of Bushen Yangxue Mingmu formula on experi-mental retinal degeneration, thus to provide evidence for further clinical application. METHODS C57BL/6N mice were induced retinitis degeneration with sodium iodate and divided randomly into two groups:distilled water group, Bushen Yangxue Mingmu group. The two groups of mice were gavaged with distilled water, Bushen Yangxue Mingmu formula respectively from day 1 after sodium iodate treatment. Ten normal mice were used as the normal control without any treatment. On day 7, 14 after treatment, retinal electrophysiology examination ,histopathology study and TUNEL test were performed to evaluate the retinopathy. RESULTS On day 7 after treatment, analysed the amplitude of dark adapted electroretinogram (d-ERG), oscillatory potentials (OPs) and light adapted electroretinogram (l-ERG) in the distilled water group and Bushen Yangxue Mingmu group, and there was no significant difference (P>0.05). The retinal pigment cells in distilled water group and Bushen Yangxue Mingmu group were decreased, and the outer nuclear layers appeared disorder and wavy change, but there was less retinopathy in Bushen Yangxue Mingmu group than distilled water group. The lesion grades were 3.18 ±0.603 and 3.64 ±0.924, and the difference among the two groups was significant (P<0.01). The apoptosis rates determined by TUNEL were 36.2%and 60.7%in the two groups, and the difference among them was significant(P<0.05). On day 14, there was significant difference between the two groups in amplitude of retinal d-ERG, OPs, l-ERG (P<0.05).Retinal outer nuclear layer cells in each group was also decreased, but the degree and scope of lesions than the day 7.The lesion grades were 2.09 ±0.539 and 2.73 ±0.647 respectively in Bushen Yangxue Mingmu group and distilled water group, there was significant difference among the groups (P<0.05). In TUNEL test, it was shown that there were less apoptosis cells in the treament groups, and no apoptosis was found in the outer nuclear layer which was found in the distilled water group. The apoptosis rated in the two was 28.8% and 51.5%, which Bushen Yangxue Mingmu group was significantly less than that of distilled water group (P<0.05). CONCLUSIONS Bushen Yangxue Mingmu formula had intervention effect on the experimental retinal degeneration, and could allevi-ate the severity of retinopathy, decrease apoptosis of retinal pigment epithelial cells and photoreceptor cells, though its mechanism needs further study.