CAJ | 학술논문

近年来，固体核磁共振被广泛应用于膜蛋白、纤维化蛋白等体系的结构和功能研究。在固体核磁共振实验中，快速魔角旋转或高功率射频场照射等实验条件将导致样品发热。生物样品发热能导致严重的后果，例如样品温度的快速升高，信号分辨率、信噪比的降低，发热严重时甚至导致样品的不可逆损坏。近年来，人们对样品发热问题进行了一些研究，发现通过优化样品制备条件或固体核磁共振实验条件，以及改进探头设计等手段，可以在一定程度上减轻样品发热。该文主要综述了生物固体核磁共振研究中导致样品发热的原因和减轻样品发热的方法。
근년래，고체핵자공진피엄범응용우막단백、섬유화단백등체계적결구화공능연구。재고체핵자공진실험중，쾌속마각선전혹고공솔사빈장조사등실험조건장도치양품발열。생물양품발열능도치엄중적후과，례여양품온도적쾌속승고，신호분변솔、신조비적강저，발열엄중시심지도치양품적불가역손배。근년래，인문대양품발열문제진행료일사연구，발현통과우화양품제비조건혹고체핵자공진실험조건，이급개진탐두설계등수단，가이재일정정도상감경양품발열。해문주요종술료생물고체핵자공진연구중도치양품발열적원인화감경양품발열적방법。
Magic-angle-spinning (MAS) solid-state NMR studies of biomolecules involve application of rapid sample rotation and radiofrequency pulsing, both of which can increase the sample temperature significantly, causing distorted spectra, sample dehydration and even sample degradation. In this review, the mechanisms leading to bio-sample heating in MAS solid-state NMR experiments are first introduced. The importance of sample temperature monitoring is then emphasized. Finally, we present the methods that can be used to to alleviate the problem, including optimization of sample preparation, selecting optimal NMR parameters, and improving NMR spectrometer hardware such as the probes.