CAJ | 학술논문

木质纤维素水解液中的毒性副产物对酿酒细胞具有抑制作用，采用氧化还原电位(oxidoreduction potential, ORP)调控，能够提高细胞对抑制物的耐受性。考察了代表性抑制物乙酸、糠醛及苯酚对酵母细胞生长和代谢的影响，并通过添加氧化剂赤血盐(K3[Fe(CN)6])和还原剂二硫苏糖醇(DTT)调节发酵液ORP初始值在（305±5）mV、（157±8）mV及（?150±5）mV，研究不同ORP条件下细胞对抑制物的耐受性影响。结果表明，氧化态能提高生物量并缩短发酵时间。除苯酚外，氧化态也利于提高乙醇的收率。对细胞保护剂甘油合成的方面，氧化态和还原态都会促进甘油合成。在水解液脱毒方面，氧化态表现比还原态要好，因为氧化态促进生物量积累，有利于通过细胞自身代谢脱毒。
목질섬유소수해액중적독성부산물대양주세포구유억제작용，채용양화환원전위(oxidoreduction potential, ORP)조공，능구제고세포대억제물적내수성。고찰료대표성억제물을산、강철급분분대효모세포생장화대사적영향，병통과첨가양화제적혈염(K3[Fe(CN)6])화환원제이류소당순(DTT)조절발효액ORP초시치재（305±5）mV、（157±8）mV급（?150±5）mV，연구불동ORP조건하세포대억제물적내수성영향。결과표명，양화태능제고생물량병축단발효시간。제분분외，양화태야리우제고을순적수솔。대세포보호제감유합성적방면，양화태화환원태도회촉진감유합성。재수해액탈독방면，양화태표현비환원태요호，인위양화태촉진생물량적루，유리우통과세포자신대사탈독。
A broad range of inhibitors in hydrolysate of lignocellulose, including weak acids, furan derivatives and phenolic compounds restrain yeast growth and subsequent fermentation. The effect of oxido-reduction potential (ORP) regulation on yeast cell tolerance to the presence of acetic acid, furfural and phenol was investigated. Cell growth and metabolite distribution changed under different ORP levels [(305±5) mV, (157±8) mV and (?150±5) mV] by feeding oxidant (potassium ferricyanide, K3[Fe(CN)6]) and reductant (dithiotreitol, DTT). Compared to the control group, cell growth was enhanced, and ethanol yield increased except for phenol group. The yield of glycerol was enhanced under both oxidizing and reducing conditions, since glycerol was a main protective molecule in yeast. High ORP level facilitated detoxification of inhibitors thanks to high biomass accumulation.