CAJ | 학술논문

以稀释糖蜜为底物，通过厌氧接触式发酵制氢反应器(ACR)的启动和运行，考察了ACR在不同进水COD浓度下的运行特性。结果表明，当HRT=6 h，进水COD浓度从7000 mg·L?1提升至11000 mg·L?1时，反应器仍能稳定运行，并维持乙醇型发酵类型。随着底物浓度的增加，系统的比产氢速率从COD 7000 mg·L?1时的2.43 m3·(m3·d)?1提高到COD11000 mg·L?1时的3.51 m3·(m3·d)?1，而活性污泥的比产氢速率在COD 为9000 mg·L?1时最高，为10.71 mol H2·(kg VSS·d)?1。聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)分析结果表明，产氢发酵产乙醇菌群为ACR系统中的主要产氢功能菌群，且随着进水COD浓度的增加，以Ethanoligenens harbinense YUAN-3为代表的产氢菌群的优势度显著增强，但丙酸发酵菌属Propionicimonas sp. F6也开始富集。
이희석당밀위저물，통과염양접촉식발효제경반응기(ACR)적계동화운행，고찰료ACR재불동진수COD농도하적운행특성。결과표명，당HRT=6 h，진수COD농도종7000 mg·L?1제승지11000 mg·L?1시，반응기잉능은정운행，병유지을순형발효류형。수착저물농도적증가，계통적비산경속솔종COD 7000 mg·L?1시적2.43 m3·(m3·d)?1제고도COD11000 mg·L?1시적3.51 m3·(m3·d)?1，이활성오니적비산경속솔재COD 위9000 mg·L?1시최고，위10.71 mol H2·(kg VSS·d)?1。취합매련식반응-변성제도응효전영(PCR-DGGE)분석결과표명，산경발효산을순균군위ACR계통중적주요산경공능균군，차수착진수COD농도적증가，이Ethanoligenens harbinense YUAN-3위대표적산경균군적우세도현저증강，단병산발효균속Propionicimonas sp. F6야개시부집。
The operation performance of fermentative hydrogen production in anaerobic contact reactor (ACR) at different influent COD concentrations was investigated. The ACR could be kept at steady-state with ethanol-type fermentation, as influent chemical oxygen demand (COD) increased from 7000 mg·L?1 to 11000 mg·L?1 with constant HRT of 6 h. Specific hydrogen production of the ACR system increased from 2.43 m3·(m3·d)?1 to 3.51 m3·(m3·d)?1 as influent COD increased from 7000 mg·L?1 to 11000 mg·L?1, while specific hydrogen production of activated sludge peaked at 10.71 mol H2·(kg VSS·d)?1 at influent COD of 9000 mg·L?1. The results of polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) profiles showed that hydrogen-producing ethanol fermentative bacteria were dominant in the ACR. As influent COD concentration increased, Ethanoligenens harbinense YUAN-3 became more abundant, and propionate fermentative bacteria, i.e. Propionicimonas sp. F6 also started to be enriched.