CAJ | 학술논문

从中原油田毛8区块油田采出液中分离得到一株多环芳烃（PAHs）降解菌吉2，根据形态观察、生理生化、16S rRNA基因、管家基因和DNA-DNA同源性分析判断，吉2属于一株Microbacterium新种。研究发现，该菌能在萘和芘为唯一碳源的无机盐培养基中生长，也能够降解萘、菲、蒽和芘的混合PAHs。7 d对这4种PAHs的降解率分别达到了61.4%、86.6%、69.9%和18.6%。吉2对原油有很好的降解及降黏作用，7 d原油的降解速率达到了134 mg·d?1，黏度降低了29.3%。傅里叶变换红外光谱、族组分和气相色谱-质谱分析显示吉2能够优先利用原油中含PAHs的芳香烃和胶质等组分，原油中萘系列、菲系列、噻吩系列、芴系列、?系列、C21-三芳甾醇、芘和苯并(a)芘的相对含量都有一定程度的降低。实验结果表明吉2具有修复PAHs和原油污染的水体或土壤环境的能力，为微生物修复PAHs污染和原油污染提供了一种可行的途径。
종중원유전모8구괴유전채출액중분리득도일주다배방경（PAHs）강해균길2，근거형태관찰、생리생화、16S rRNA기인、관가기인화DNA-DNA동원성분석판단，길2속우일주Microbacterium신충。연구발현，해균능재내화비위유일탄원적무궤염배양기중생장，야능구강해내、비、은화비적혼합PAHs。7 d대저4충PAHs적강해솔분별체도료61.4%、86.6%、69.9%화18.6%。길2대원유유흔호적강해급강점작용，7 d원유적강해속솔체도료134 mg·d?1，점도강저료29.3%。부리협변환홍외광보、족조분화기상색보-질보분석현시길2능구우선이용원유중함PAHs적방향경화효질등조분，원유중내계렬、비계렬、새분계렬、물계렬、?계렬、C21-삼방치순、비화분병(a)비적상대함량도유일정정도적강저。실험결과표명길2구유수복PAHs화원유오염적수체혹토양배경적능력，위미생물수복PAHs오염화원유오염제공료일충가행적도경。
A PAH-degrading strain JI 2 was isolated from oilfield produced water in Mao 8 block of Zhongyuan Oilfield. According to analysis of morphological observation, physiological and biochemical test, 16S rRNA gene, house-keeping genes and DNA-DNA hybridization, JI 2 was considered to represent a novel species of the genus Microbacterium. The strain could not only grow in inorganic salt culture medium and utilize naphthalene and pyrene as the sole carbon source, but also degrade mixed naphthalene, phenanthrene, anthracene and pyrene. The four PAHs degradation rates could reach 61.4%, 86.6%, 69.9% and 18.6%, respectively after 7 d. The viscosity of crude oil treated with JI 2 decreased by 29.3% and degradation rate was 134 mg·d?1. Fourier transform infrared spectra, group composition and GC-MS analysis of the crude oil treated with JI 2 showed that the strain tended to utilize PAHs in aromatic hydrocarbons and resins in crude oil. The relative contents of naphthalene series, phenanthrenseries, thiophene series, fluorene series and chrysene series, C21-triaromatic steroid, pyrene, and benz(a)pyrene decreased after degradation. JI 2 had the capacity to remediate water and soil environment contaminated by PAHs and oil, and provided a feasible way for bioremediation of PAHs and oil pollution.