CAJ | 학술논문

目的：检测不同时段肝郁脾虚证模型大鼠外周红细胞、骨髓红细胞及造血细胞调节因子,从血液系统探讨其变化规律。方法采用束缚应激＋夹尾激怒＋过度疲劳＋饮食失节方法建立肝郁脾虚证大鼠模型,检测空白对照组和模型2、6、9周组大鼠外周红细胞、骨髓有核红细胞、干细胞因子及促红细胞生成素。结果与空白对照组比较,模型2周组大鼠红细胞压积和晚幼红细胞数量升高(P＜0.05),模型6周组大鼠促红细胞生成素降低(P＜0.01),模型9周组大鼠红细胞计数、血红蛋白含量、红细胞压积和促红细胞生成素降低(P＜0.05)；与模型2周组比较,模型6、9周组大鼠红细胞计数、血红蛋白含量、红细胞压积、早幼红细胞比例、晚幼红细胞比例和促红细胞生成素降低(P＜0.05,P＜0.01),模型9周组有核红细胞比例降低(P＜0.05)；干细胞因子差异无统计学意义。结论肝郁脾虚证大鼠遭受应激刺激时虽然短期能使红细胞的数量增加以对抗伤害因素作用,但长期作用会导致机体贫血,可能与促红细胞生成素减少有关。
목적：검측불동시단간욱비허증모형대서외주홍세포、골수홍세포급조혈세포조절인자,종혈액계통탐토기변화규률。방법채용속박응격＋협미격노＋과도피로＋음식실절방법건립간욱비허증대서모형,검측공백대조조화모형2、6、9주조대서외주홍세포、골수유핵홍세포、간세포인자급촉홍세포생성소。결과여공백대조조비교,모형2주조대서홍세포압적화만유홍세포수량승고(P＜0.05),모형6주조대서촉홍세포생성소강저(P＜0.01),모형9주조대서홍세포계수、혈홍단백함량、홍세포압적화촉홍세포생성소강저(P＜0.05)；여모형2주조비교,모형6、9주조대서홍세포계수、혈홍단백함량、홍세포압적、조유홍세포비례、만유홍세포비례화촉홍세포생성소강저(P＜0.05,P＜0.01),모형9주조유핵홍세포비례강저(P＜0.05)；간세포인자차이무통계학의의。결론간욱비허증대서조수응격자격시수연단기능사홍세포적수량증가이대항상해인소작용,단장기작용회도치궤체빈혈,가능여촉홍세포생성소감소유관。
Objective To test red cells of bone marrow and hematopoietic cells of rats with liver depression and spleen deficiency syndrome in different time period;To discuss the change rules in the blood system. Methods Rat models with liver depression and spleen deficiency syndrome were made by composite method of anger by restraint stress, clipping tails, over fatigue, and abnormal diet. The hemogram, red cells of bone marrow, marrow nucleated red blood cells, stem cell factor, and erythropoietin of male rats in normal group and model group were detected at 2nd, 6th and 9th weeks. Results Compared with normal group, hematokrit and metarubricyte in model group of 2 weeks was higher in the model group (P<0.05);erythropoietin in model group of 6 weeks decreased (P<0.01);red cell counts, red protein level, hemoglobin, hematokrit, and erythropoietin in model group of 9 weeks were lower (P<0.05);red cell counts, hemoglobin, hematokrit, prorubricyte, metarubricyte, and erythropoietin in model group of 6 and 9 weeks were significantly lower than those in model group of 2 weeks (P<0.05, P<0.01). The number of marrow nucleated red blood cells in model group of 2 weeks decreased (P<0.05). Stem cell factor showed no significant difference. Conclusion The rats with liver depression and spleen deficiency syndrome can increase the number of red blood cells in a short time when they suffer from stress stimulation, so that they can fight the damage factors. But long-term stress stimulation will lead the rats to anemia, which may be associated with the reduction of erythropoietin.