CAJ | 학술논문

目的：建立肾石通颗粒的高效液相色谱特征图谱。方法采用Thermo Hypersil GOLD-C18色谱柱(4.6 mm×25.0 mm,5μm),以乙腈-0.2%磷酸水溶液为洗脱剂进行梯度洗脱(0～20 min,5%→10%乙腈；20～40 min,10%→12%乙腈；40～60 min,12%→14%乙腈；60～90 min,14%→20%乙腈；90～120 min,20%→28%乙腈),检测波长280 nm,流速1.0 mL/min,柱温30℃,分析时间120 min。结果根据10批样品建立了肾石通颗粒的高效液相色谱特征图谱,确定了27个共有峰,其中包含丹参、金钱草等6味药的特征峰。结论该法灵敏度高、稳定性和重复性良好,可用于肾石通颗粒的质量控制。
목적：건립신석통과립적고효액상색보특정도보。방법채용Thermo Hypersil GOLD-C18색보주(4.6 mm×25.0 mm,5μm),이을정-0.2%린산수용액위세탈제진행제도세탈(0～20 min,5%→10%을정；20～40 min,10%→12%을정；40～60 min,12%→14%을정；60～90 min,14%→20%을정；90～120 min,20%→28%을정),검측파장280 nm,류속1.0 mL/min,주온30℃,분석시간120 min。결과근거10비양품건립료신석통과립적고효액상색보특정도보,학정료27개공유봉,기중포함단삼、금전초등6미약적특정봉。결론해법령민도고、은정성화중복성량호,가용우신석통과립적질량공제。
Objective To establish the HPLC characteristic chromatogram of Shenshitong Granules. Methods The chromatographie fingerprints were obtained through Thermo Hypersil GOLD-C18 column (4.6 mm×250 mm, 5μm) with the gradient elution solvent system composed of acetonitrile-0.2% phosphoric acid (0-20 min, 5%→10% acetonitrile;20-40 min, 10%→12%acetonitrile;40-60 min, 12%→14%acetonitrile;60-90 min, 14%→20%acetonitrile;90-120 min, 20%→28%acetonitrile). The detective wavelength was set at 280 nm;the flow rate was 1.0 mL/min;the column temperature was maintained at 30 ℃;the analysis time was 120 min. Results The HPLC characteristic chromatogram was built on basis of 10 batches of Shenshitong Granules, including 27 common peaks which contain the characteristic peaks of 6 Chinese herbal medicines, such as Radix Salvia Miltiorrhizae, Herba Lysimachiae, etc. Conclusion The established HPLC fingerprint has high sensitivity and good repeatability, and can be available for quality evaluation of Shenshitong Granules.