当代化工
噹代化工
당대화공
CONTEMPORARY CHEMICAL INDUSTRY
2015年
2期
426-428
,共3页
妇炎康冲剂%隐丹参酮%丹参酮Ⅰ%丹参酮ⅡA%穿心莲内酯%脱水穿心莲内酯%RP-HPLC
婦炎康遲劑%隱丹參酮%丹參酮Ⅰ%丹參酮ⅡA%穿心蓮內酯%脫水穿心蓮內酯%RP-HPLC
부염강충제%은단삼동%단삼동Ⅰ%단삼동ⅡA%천심련내지%탈수천심련내지%RP-HPLC
Fuyankang granules%Cryptotanshinone%Tanshinone Ⅰ%Tanshinone Ⅱ A%Andrographolide%Dehydroandrographolide%RP-HPLC
建立 RP-HPLC 同时测定妇炎康冲剂中5种成分的含量。采用 Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm)色谱柱；以甲醇-水为流动相梯度洗脱；流速1.0 mL/min；柱温26℃；检测波长254 nm。隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA、穿心莲内酯、脱水穿心莲内酯分别在0.5068~360.2、0.2912~208、0.2576~184、0.5488~392、0.3444~246μg·mL-1范围内与峰面积呈很好的线性关系（r=1）；方法平均回收率为98.93%，100.83%，99.38%，100.2%，99.97%。本法操作简便、结果可靠，可以很好的用于妇炎康冲剂的质量控制。
建立 RP-HPLC 同時測定婦炎康遲劑中5種成分的含量。採用 Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm)色譜柱；以甲醇-水為流動相梯度洗脫；流速1.0 mL/min；柱溫26℃；檢測波長254 nm。隱丹參酮、丹參酮Ⅰ、丹參酮ⅡA、穿心蓮內酯、脫水穿心蓮內酯分彆在0.5068~360.2、0.2912~208、0.2576~184、0.5488~392、0.3444~246μg·mL-1範圍內與峰麵積呈很好的線性關繫（r=1）；方法平均迴收率為98.93%，100.83%，99.38%，100.2%，99.97%。本法操作簡便、結果可靠，可以很好的用于婦炎康遲劑的質量控製。
건립 RP-HPLC 동시측정부염강충제중5충성분적함량。채용 Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm)색보주；이갑순-수위류동상제도세탈；류속1.0 mL/min；주온26℃；검측파장254 nm。은단삼동、단삼동Ⅰ、단삼동ⅡA、천심련내지、탈수천심련내지분별재0.5068~360.2、0.2912~208、0.2576~184、0.5488~392、0.3444~246μg·mL-1범위내여봉면적정흔호적선성관계（r=1）；방법평균회수솔위98.93%，100.83%，99.38%，100.2%，99.97%。본법조작간편、결과가고，가이흔호적용우부염강충제적질량공제。
A RP-HPLC method for simultaneous determination of cryptotanshinone, tanshinone Ⅰand tanshinone ⅡA, andrographolide and dehydroandrographolide in Fuyankang granules was developed . The analysis was performed on a C18(250 mm×4.6 mm,5μm) column.The mobile phase was composed of methamol and water in gradient elution mode with at a flow rate of 1.0 ml/min.The column temperature was 26 ℃ and the UV detection wavelength was 254 nm. The results show that, the linear ranges of cryptotanshinone, tanshinone Ⅰ,tanshinone ⅡA , andrographolide and dehydroandrographolide are 0.506 8 ~360.2,0.291 2~208,0.257 6~184,0.548 8~392,0.344 4~246μg·mL-1 (r=1), respectively.The average recoveries (n=5) of cryptotanshinone, tanshinone Ⅰ,tanshinone ⅡA , andrographolide and dehydroandrographolide are 98.93%,100.83%,99.38%,100.2% and 99.97%, respectively. The method is convenient and accurate.It can be used for quality control in production of Fuyankang granules.
