CAJ | 학술논문

探讨酒中生物胺对小鼠行为学及脑中5-羟色胺含量的影响。建立48%vol食用酒精对小鼠饮后上头和醉酒的实验模型，确定旷场实验、爬杆时间和翻正反射在模型中的评价作用；配制不同浓度的酪胺、组胺、苯乙胺、色胺、腐胺及混合胺的48%食用酒精溶液，连续灌胃5 d，进行行为学评价，最后一次灌胃后检测脑中5-羟色胺含量。结果表明，小鼠的上头灌胃剂量为10.0 mL/kg·bw，醉酒灌胃剂量为15.0 mL/kg·bw，旷场试验、爬杆实验、翻正反射相互结合，可反映小鼠的行为学表现；低剂量、低浓度生物胺灌胃的行为学反应与48%vol酒精灌胃无显著性差异（P＞0.05），高剂量、高浓度生物胺灌胃时，酪胺和苯乙胺使小鼠活动兴奋，不易醉酒，而组胺和混合胺加剧醉酒的发生，其他结果无显著性差异；酪胺灌胃30 min后，小鼠脑内5-HT含量明显高于空白组和模型组（P＜0.05），组胺组接近正常小鼠，而混合胺明显低于空白组和模型组（P＜0.05），色胺、苯乙胺和腐胺与模型组无显著性差异，均低于正常小鼠（P＜0.05）。
탐토주중생물알대소서행위학급뇌중5-간색알함량적영향。건립48%vol식용주정대소서음후상두화취주적실험모형，학정광장실험、파간시간화번정반사재모형중적평개작용；배제불동농도적락알、조알、분을알、색알、부알급혼합알적48%식용주정용액，련속관위5 d，진행행위학평개，최후일차관위후검측뇌중5-간색알함량。결과표명，소서적상두관위제량위10.0 mL/kg·bw，취주관위제량위15.0 mL/kg·bw，광장시험、파간실험、번정반사상호결합，가반영소서적행위학표현；저제량、저농도생물알관위적행위학반응여48%vol주정관위무현저성차이（P＞0.05），고제량、고농도생물알관위시，락알화분을알사소서활동흥강，불역취주，이조알화혼합알가극취주적발생，기타결과무현저성차이；락알관위30 min후，소서뇌내5-HT함량명현고우공백조화모형조（P＜0.05），조알조접근정상소서，이혼합알명현저우공백조화모형조（P＜0.05），색알、분을알화부알여모형조무현저성차이，균저우정상소서（P＜0.05）。
In this study, the effects of biogenic amine in liquor on mice's drunken behavior and the 5-HT content in their brains were investigat-ed. Firstly, the experimental models of dizzy&drunken mice (intragastric administration of 48%vol edible alcohol) were established, and the evaluation roles of field test, pole-climbing time, and righting reflex in the models were determined. 48%vol edible alcohol was mixed with dif-ferent concentration of histamine, tyramine, phenylethylamine, putrescine, serotonin, and mixed amine, behavior evaluation was carried out af-ter 5 d continuous intragastric administration, and 5-HT content was measured after the final intragastric administration. The results showed that, the intragastric administration doses for mice's dizzy and mice's drunkenness were 10.0 mL/kg·bw and 15.0 mL/kg·bw respectively, and the combination of field test, pole-climbing time,and righting reflex could reflect mice's behavior;there was no significant difference in mice's behavior between intragastric administration of low-dose&low-concentration biogenic amine and of 48%alcohol (P>0.05), however, gastric administration of high-dose&high-concentration biogenic amine would excite mice but not easily result in mice’s drunkenness (histamine and phenylethylamine), and tyramine and mixed amine would aggravate mice’s drunkenness, there was no significant difference for other biogenic amine;30 mins after gastric administration of histamine, mice's 5-HT content was obviously higher than that in blank group and model group (P<0.05), gastric administration of tyramine group was close to normal mice group, and gastric administration of mixed amine group was obvi-ously lower than blank group and model group (P<0.05), and phenylethylamine, putrescine and serotonin groups had no significant difference with model group (5-HT content in all groups lower than normal mice group (P<0.05)).