CAJ | 학술논문

采用多聚酶链式反应结合变性梯度凝胶电泳指纹分析技术（PCR-DGGE）对不同质量窖泥中古菌群落结构进行分析，说明了不同质量窖泥古菌种群的复杂性，再次印证了产甲烷菌是窖泥中古菌的优势菌，并在此基础上揭示了窖泥中产甲烷菌种属上的多样性。发现了以最优质窖泥为参照，窖泥质量与其古菌群落间的相似性指数总体呈正相关这一规律，为今后研究窖泥培养、养护和应用提供了理论依据。
채용다취매련식반응결합변성제도응효전영지문분석기술（PCR-DGGE）대불동질량교니중고균군락결구진행분석，설명료불동질량교니고균충군적복잡성，재차인증료산갑완균시교니중고균적우세균，병재차기출상게시료교니중산갑완균충속상적다양성。발현료이최우질교니위삼조，교니질량여기고균군락간적상사성지수총체정정상관저일규률，위금후연구교니배양、양호화응용제공료이론의거。
In this study, the structures of archaea communities in pit mud of different quality were analyzed by PCR-DGGE. The analytic re-sults proved the complexity of archaea communities in pit mud of different quality. And it also proved that methanogenic bacteria was the domi-nant archaeal in pit mud. Besides, the analytic results revealed the diversity of methanogenic bacteria species. It was found that, with reference to the best pit mud, the quality of pit mud was positively correlated to archaeal community similarity indexes. Those findings provided theoreti-cal evidences for further research on pit mud culture, maintenance and application.