酿酒科技
釀酒科技
양주과기
LIQUOR-MAKING SCIENCE & TECHNOLOGY
2015年
2期
114-117
,共4页
气相色谱法%双内标法%己酸%己酸乙酯%白酒
氣相色譜法%雙內標法%己痠%己痠乙酯%白酒
기상색보법%쌍내표법%기산%기산을지%백주
GC%double-internal standard method%caproic acid%ethyl caproate%Baijiu(liquor)
建立毛细管气相色谱法同时测定发酵液中己酸和己酸乙酯的方法。发酵液经甲酸酸化后，用正己烷萃取，以2-乙基正丁酸和乙酸正丁酯作为双内标，采用Rtx-Wax毛细管色谱柱。结果表明，己酸和己酸乙酯浓度分别在100～1000 mg/100 mL（r=0.9991）、10～100 mg/100 mL（r=0.9995）质量浓度范围内线性关系良好，平均回收率（n=9）分别为101.74%、94.96%，RSD分别为0.85%、0.38%。该方法简单、灵敏、准确度高，可用于同时测定发酵液中己酸和己酸乙酯的含量。
建立毛細管氣相色譜法同時測定髮酵液中己痠和己痠乙酯的方法。髮酵液經甲痠痠化後，用正己烷萃取，以2-乙基正丁痠和乙痠正丁酯作為雙內標，採用Rtx-Wax毛細管色譜柱。結果錶明，己痠和己痠乙酯濃度分彆在100～1000 mg/100 mL（r=0.9991）、10～100 mg/100 mL（r=0.9995）質量濃度範圍內線性關繫良好，平均迴收率（n=9）分彆為101.74%、94.96%，RSD分彆為0.85%、0.38%。該方法簡單、靈敏、準確度高，可用于同時測定髮酵液中己痠和己痠乙酯的含量。
건립모세관기상색보법동시측정발효액중기산화기산을지적방법。발효액경갑산산화후，용정기완췌취，이2-을기정정산화을산정정지작위쌍내표，채용Rtx-Wax모세관색보주。결과표명，기산화기산을지농도분별재100～1000 mg/100 mL（r=0.9991）、10～100 mg/100 mL（r=0.9995）질량농도범위내선성관계량호，평균회수솔（n=9）분별위101.74%、94.96%，RSD분별위0.85%、0.38%。해방법간단、령민、준학도고，가용우동시측정발효액중기산화기산을지적함량。
In this study, the determination of caproic acid and ethyl caproate content simultaneously in fermenting liquid by GC and double-in-ternal standard method had been developed. The fermenting liquid was extracted with n-hexane after adding formic acid, then 2-ethylbutyric acid and n-butyl acetate were adopted as double internal standards, and GC was performed on a Rtx-Wax column. The results showed that, the calibration curves of caproic acid and ethyl caproate in the concentration range of 100~1000 mg/100 mL(r=0.9991)and 10~100 mg/100 mL (r=0.9995)respectively, their mean recoveries (n=9) were 101.74%and 94.96%respectively, and RSD were 0.85%and 0.38%respectively. Such method was simple,rapid,accurate and suitable for simultaneous determination of caproic acid and ethyl caproate content in fermenting liquid.
