CAJ | 학술논문

目的：观察缺血后处理对大鼠睾丸缺血再灌注损伤的影响。方法60只成年雄性SD大鼠随机分为对照组、缺血再灌注组和缺血后处理组，每组20只。对照组行左侧睾丸假手术。缺血再灌注组予左侧睾丸扭转720度，2h后复位。缺血后处理组予左侧睾丸扭转720度，2h后行缺血后处理，即先将睾丸复位，血流再灌注10s，然后将睾丸再次扭转720度，使睾丸缺血10s，反复6个循环，最后睾丸复位，血流持续灌注。复位后4h，每组处死大鼠10只，行左侧睾丸切除术，测定睾丸组织丙二醛水平。复位后3个月，各组处死剩余的大鼠，行左侧睾丸切除术，分析睾丸生精功能。结果睾丸组织丙二醛含量缺血后灌注组>缺血后处理组>处理组[（2.73±0.34）、（1.86±0.29）、（1.45±0.20）mmol/mg]（P<0.05），睾丸生精功能对照组>缺血后处理组>缺血后灌注组[（22.03±1.97）、（15.13±1.81）、（2.14±0.42）×106]（P<0.05）。结论缺血后处理对睾丸缺血再灌注损伤有保护作用，其作用机制可能与降低活性氧水平有关。
목적：관찰결혈후처리대대서고환결혈재관주손상적영향。방법60지성년웅성SD대서수궤분위대조조、결혈재관주조화결혈후처리조，매조20지。대조조행좌측고환가수술。결혈재관주조여좌측고환뉴전720도，2h후복위。결혈후처리조여좌측고환뉴전720도，2h후행결혈후처리，즉선장고환복위，혈류재관주10s，연후장고환재차뉴전720도，사고환결혈10s，반복6개순배，최후고환복위，혈류지속관주。복위후4h，매조처사대서10지，행좌측고환절제술，측정고환조직병이철수평。복위후3개월，각조처사잉여적대서，행좌측고환절제술，분석고환생정공능。결과고환조직병이철함량결혈후관주조>결혈후처리조>처리조[（2.73±0.34）、（1.86±0.29）、（1.45±0.20）mmol/mg]（P<0.05），고환생정공능대조조>결혈후처리조>결혈후관주조[（22.03±1.97）、（15.13±1.81）、（2.14±0.42）×106]（P<0.05）。결론결혈후처리대고환결혈재관주손상유보호작용，기작용궤제가능여강저활성양수평유관。
Objective To investigate the effect of ischemic post-conditioning on testicular ischemia-reperfusion (IR) injury in rats. Methods Sixty adult male Sprague-Dawley rats were randomly divided into three groups: con-trol group, IR group, and ischemic post-conditioning group, with 20 rats in each group. Control group underwent a sham operation on the left testis. In IR group, the left testis was rotated 720° for 2h, then detorsion was per-formed. In ischemic post-conditioning group, the left testis was rotated 720° for 2h, then ischemic post-conditioning was performed. First, testicular detorsion was done for 10-second blood flow reperfusion. Then, the left testis was rotated 720° again for 10-second ischemia. Such procedures were repeated 6 times. Lastly, testicular detorsion was performed for persistent blood flow reperfusion. One half of the rats in each group were sacrificed at 4h after detorsion, and left orchiectomy was done for measurement of malondialdehyde level. The remainder were killed at 3 months after detorsion, and left orchiectomy was performed for analysis of testicular spermatogenesis. Results Tes-ticular torsion-detorsion caused a significant increase in the malondialdehyde level in the testes(2.73±0.34 vs 1.45± 0.20mmol/mg, P<0.05) and a significant decrease in testicular spermatogenesis [(2.14±0.42)×106 vs (22.03±1.97)× 106, P<0.05]. Ischemic post-conditioning significantly decreased malondialdehyde level in testes (1.86±0.29 vs 2.73± 0.34mmol/mg, P<0.05), and improved testicular spermatogenesis [(15.13 ±1.81)×106 vs (2.14 ±0.42)×106, P<0.05]. Conclusion Ischemic post-conditioning has a protective effect on testicular ischemia-reperfusion injury. The effect is related to reduction in production of reactive oxygen species in testes.