浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2015年
2期
118-119,123
,共3页
郑红霞%章伟明%周红娟%张雯%于健宁%汪卫
鄭紅霞%章偉明%週紅娟%張雯%于健寧%汪衛
정홍하%장위명%주홍연%장문%우건저%왕위
小鼠%胶原诱导性关节炎%薏苡仁酯%Th17细胞%白细胞介素-17
小鼠%膠原誘導性關節炎%薏苡仁酯%Th17細胞%白細胞介素-17
소서%효원유도성관절염%의이인지%Th17세포%백세포개소-17
mice%collagen induced arthritis coixenolide%Th17 cells%interleukin-17
目的:研究薏苡仁酯对胶原诱导性关节炎(CIA)小鼠体内Th17细胞及其细胞因子白细胞介素-17(IL-17A)的作用。方法健康SPF级DBA/17~8周雄性小鼠20只,测定造模对照组(经生理盐水治疗后CIA小鼠)、薏苡仁酯组(经薏苡仁酯注射液治疗后CIA小鼠)小鼠脾脏Th17细胞及血清中IL-17变化。应用流式细胞术(FCM)分析小鼠脾脏中Th17细胞的比例,采用酶联免疫吸附试验(ELISA)检测小鼠血清中IL-17A含量。结果经薏苡仁酯注射液治疗后,薏苡仁酯组CIA小鼠脾脏中Th17细胞比例较造模对照组显著降低(1.82%比4.99%,P<0.05),小鼠血清中IL-17A的含量也明显低于造模对照组[(3.54±0.61)pg/mL比(8.31±0.25)pg/mL,P<0.05]。结论薏苡仁酯对CIA小鼠Th17细胞具有分化作用。
目的:研究薏苡仁酯對膠原誘導性關節炎(CIA)小鼠體內Th17細胞及其細胞因子白細胞介素-17(IL-17A)的作用。方法健康SPF級DBA/17~8週雄性小鼠20隻,測定造模對照組(經生理鹽水治療後CIA小鼠)、薏苡仁酯組(經薏苡仁酯註射液治療後CIA小鼠)小鼠脾髒Th17細胞及血清中IL-17變化。應用流式細胞術(FCM)分析小鼠脾髒中Th17細胞的比例,採用酶聯免疫吸附試驗(ELISA)檢測小鼠血清中IL-17A含量。結果經薏苡仁酯註射液治療後,薏苡仁酯組CIA小鼠脾髒中Th17細胞比例較造模對照組顯著降低(1.82%比4.99%,P<0.05),小鼠血清中IL-17A的含量也明顯低于造模對照組[(3.54±0.61)pg/mL比(8.31±0.25)pg/mL,P<0.05]。結論薏苡仁酯對CIA小鼠Th17細胞具有分化作用。
목적:연구의이인지대효원유도성관절염(CIA)소서체내Th17세포급기세포인자백세포개소-17(IL-17A)적작용。방법건강SPF급DBA/17~8주웅성소서20지,측정조모대조조(경생리염수치료후CIA소서)、의이인지조(경의이인지주사액치료후CIA소서)소서비장Th17세포급혈청중IL-17변화。응용류식세포술(FCM)분석소서비장중Th17세포적비례,채용매련면역흡부시험(ELISA)검측소서혈청중IL-17A함량。결과경의이인지주사액치료후,의이인지조CIA소서비장중Th17세포비례교조모대조조현저강저(1.82%비4.99%,P<0.05),소서혈청중IL-17A적함량야명현저우조모대조조[(3.54±0.61)pg/mL비(8.31±0.25)pg/mL,P<0.05]。결론의이인지대CIA소서Th17세포구유분화작용。
Objective To explore the effect of coixenolide on T helper cell 17 (Th17) and its cytokine inter-leukin-17 (IL-17A) in mice with collagen-induced arthritis (CIA). Methods Twenty DBA/1 mice at age of 7-8 weeks were used to build CIA model, then they were randomly divided into model group and coixenolide-treated group, with 10 mice in each group. Mice in coixenolide-treated group was injected with 25 ml/kg coixenolide and mice in model group were given injection of equivalent normal saline. The proportion of Th17 cells in the spleen of mice and serum IL-17A were determined by flow cytometry and enzyme-linked immunosorbent assay(ELISA), respectively. Results The proportion of Th17 in the spleen of mice in coixenolide-treated group was significantly lower than that in model group (1.82% vs 4.99%, P<0.05). The serum IL-17 A of mice in coixenolide-treated group was significantly lower than that in control group [(3.54±0.61)pg/mL vs (8.31±0.25)pg/mL,P<0.05]. Conclu-sion Coixenolide has inhibitory effect on differentiation of Thl7 cells in CIA mice.