CAJ | 학술논문

目的：研究薏苡仁酯对胶原诱导性关节炎（CIA）小鼠体内Th17细胞及其细胞因子白细胞介素-17（IL-17A）的作用。方法健康SPF级DBA/17~8周雄性小鼠20只，测定造模对照组（经生理盐水治疗后CIA小鼠）、薏苡仁酯组（经薏苡仁酯注射液治疗后CIA小鼠）小鼠脾脏Th17细胞及血清中IL-17变化。应用流式细胞术（FCM）分析小鼠脾脏中Th17细胞的比例，采用酶联免疫吸附试验（ELISA）检测小鼠血清中IL-17A含量。结果经薏苡仁酯注射液治疗后，薏苡仁酯组CIA小鼠脾脏中Th17细胞比例较造模对照组显著降低（1.82%比4.99%，P<0.05），小鼠血清中IL-17A的含量也明显低于造模对照组[（3.54±0.61）pg/mL比（8.31±0.25）pg/mL，P<0.05]。结论薏苡仁酯对CIA小鼠Th17细胞具有分化作用。
목적：연구의이인지대효원유도성관절염（CIA）소서체내Th17세포급기세포인자백세포개소-17（IL-17A）적작용。방법건강SPF급DBA/17~8주웅성소서20지，측정조모대조조（경생리염수치료후CIA소서）、의이인지조（경의이인지주사액치료후CIA소서）소서비장Th17세포급혈청중IL-17변화。응용류식세포술（FCM）분석소서비장중Th17세포적비례，채용매련면역흡부시험（ELISA）검측소서혈청중IL-17A함량。결과경의이인지주사액치료후，의이인지조CIA소서비장중Th17세포비례교조모대조조현저강저（1.82%비4.99%，P<0.05），소서혈청중IL-17A적함량야명현저우조모대조조[（3.54±0.61）pg/mL비（8.31±0.25）pg/mL，P<0.05]。결론의이인지대CIA소서Th17세포구유분화작용。
Objective To explore the effect of coixenolide on T helper cell 17 (Th17) and its cytokine inter-leukin-17 (IL-17A) in mice with collagen-induced arthritis (CIA). Methods Twenty DBA/1 mice at age of 7-8 weeks were used to build CIA model, then they were randomly divided into model group and coixenolide-treated group, with 10 mice in each group. Mice in coixenolide-treated group was injected with 25 ml/kg coixenolide and mice in model group were given injection of equivalent normal saline. The proportion of Th17 cells in the spleen of mice and serum IL-17A were determined by flow cytometry and enzyme-linked immunosorbent assay(ELISA), respectively. Results The proportion of Th17 in the spleen of mice in coixenolide-treated group was significantly lower than that in model group (1.82% vs 4.99%, P<0.05). The serum IL-17 A of mice in coixenolide-treated group was significantly lower than that in control group [(3.54±0.61)pg/mL vs (8.31±0.25)pg/mL,P<0.05]. Conclu-sion Coixenolide has inhibitory effect on differentiation of Thl7 cells in CIA mice.