CAJ | 학술논문

目的：探讨高血压(HTN)对糖尿病周围神经病(DPN)大鼠模型坐骨神经中髓鞘相关糖蛋白(MAG)和髓鞘碱性蛋白(MBP)表达的影响。方法大鼠随机分为正常组、DPN组和 DPN+HTN组。造模后4周检测各组血压、血糖及坐骨神经传导速度,RT PCR检测坐骨神经MAG和MBPmRNA表达。结果与正常组比较,DPN组和HTN+DPN组血糖显著增高(P<0.01),神经传导速度显著降低(P<0.01),坐骨神经 MBP和 MAG mRNA表达显著上调(P<0.01),其中 HTN+DPN组与 DPN组比较收缩压显著增高(P<0.01),神经传导速度显著降低(P<0.01),坐骨神经 MAG mRNA表达显著升高(P<0.01),MBP mRNA表达显著下降(P<0.01)。结论高血压能够上调 MAG和下调 MBP表达,抑制髓鞘形成,加剧糖尿病周围神经病的神经损伤。
목적：탐토고혈압(HTN)대당뇨병주위신경병(DPN)대서모형좌골신경중수초상관당단백(MAG)화수초감성단백(MBP)표체적영향。방법대서수궤분위정상조、DPN조화 DPN+HTN조。조모후4주검측각조혈압、혈당급좌골신경전도속도,RT PCR검측좌골신경MAG화MBPmRNA표체。결과여정상조비교,DPN조화HTN+DPN조혈당현저증고(P<0.01),신경전도속도현저강저(P<0.01),좌골신경 MBP화 MAG mRNA표체현저상조(P<0.01),기중 HTN+DPN조여 DPN조비교수축압현저증고(P<0.01),신경전도속도현저강저(P<0.01),좌골신경 MAG mRNA표체현저승고(P<0.01),MBP mRNA표체현저하강(P<0.01)。결론고혈압능구상조 MAG화하조 MBP표체,억제수초형성,가극당뇨병주위신경병적신경손상。
Objective To investigate the effects of hypertension(HTN)on the expression of myelin associated glycoprotein (MAG) and myelin basic protein(MBP)in the sciatic nerve in rats with diabetic peripheral neuropathy(DPN).Methods The rats were randomly di-vided into normal group,DPN group and HTN+DPN group.After 4 weeks of modeling,the blood sugar,blood pressure and conduc-tion velocity of sciatic nerve were measured,the mRNA expression of MBP and MAG in the sciatic nerve was detected by RT PCR. Results After 4 weeks of modeling,compared with normal group,the blood sugar were increased in DPN group and HTN+DPN group,while the conduction velocity of sciatic nerve was decreased,the mRNA expression of MAG and MBP in sciatic nerve were in-creased (P<0.01).Compared with DPN group,the blood pressure were increased in HTN+DPN group,and the conduction velocity of sciatic nerve was decreased,the mRNA expression of MAG was increased and the expression of MBP was decreased in sciatic nerve (P<0.01).Conclusion Hypertension can contribute to worsening of DPN by elevating MAG and decreasing MBP in sciatic nerve,and inhabiting myelination.