CAJ | 학술논문

目的：以对照药材技术要求为基础，以等量同质为原则，利用现代的提取制备技术，制备成质量稳定、均匀性好的苦杏仁对照提取物。通过考察不同的薄层系统，建立薄层鉴别用对照提取物的质量标准，供中药标准中薄层鉴别使用。并对其主要药效成分进行方法学考察，确保今后制备的对照提取物的一致性。方法：采用不同薄层色谱系统对苦杏仁对照提取物可替代对照药材进行定性研究；并用高效液相色谱法测定苦杏仁对照提取物中苦杏仁苷的含量。结果：在苦杏仁对照提取物薄层色谱中，展开剂：为甲苯－甲酸乙酯－甲醇－甲酸－水（6∶2∶0．4∶0．1∶0．1 ），在与对照药材色谱相应位置上，显相同颜色的斑点。HPLC测定苦杏仁对照提取物中苦杏仁苷含量，苦杏仁苷线性范围461．7～15．39 mg／L（r＝0．9995），平均回收率为101 ．1％（RSD＝2．15％），该方法准确、可靠、专属性强，重复性好。结论：苦杏仁对照提取可替代对照药材进行定性鉴别。
목적：이대조약재기술요구위기출，이등량동질위원칙，이용현대적제취제비기술，제비성질량은정、균균성호적고행인대조제취물。통과고찰불동적박층계통，건립박층감별용대조제취물적질량표준，공중약표준중박층감별사용。병대기주요약효성분진행방법학고찰，학보금후제비적대조제취물적일치성。방법：채용불동박층색보계통대고행인대조제취물가체대대조약재진행정성연구；병용고효액상색보법측정고행인대조제취물중고행인감적함량。결과：재고행인대조제취물박층색보중，전개제：위갑분－갑산을지－갑순－갑산－수（6∶2∶0．4∶0．1∶0．1 ），재여대조약재색보상응위치상，현상동안색적반점。HPLC측정고행인대조제취물중고행인감함량，고행인감선성범위461．7～15．39 mg／L（r＝0．9995），평균회수솔위101 ．1％（RSD＝2．15％），해방법준학、가고、전속성강，중복성호。결론：고행인대조제취가체대대조약재진행정성감별。
Objective:On the basis of the control reference drug technical requirements and the principle of equivalent homogenei-ty,using modern extraction and preparation technology to prepare a stable quality and good uniformity of Semen Armeniacae Ama-rum reference substance.By investigating the thin layer of different system,to establish the quality standards of thin layer identifica-tion with reference extract,for the use of thin layer identification in traditional Chinese medicine (TCM)standard.To investigate methodology and its main efficacy components to ensure the consistency.Methods:Different thin layer chromatographic system for Semen Armeniacae Amarum reference substance qualitative alternative was used to the reference drug research.An HPLC method for content determination of amygdalin in Semen Armeniacae Amarum reference substance was established.Results:In Semen Ar-meniacae Amarum reference substance in thin layer chromatography,the mobile phase was consisted of toluene- ethyl formate-methanol-formic acid and water (6∶2∶0. 4∶0.1-0.1 ),and chromatographic corresponding to the position,the same color spots.To establish an HPLC method for content determination of amygdalin in Semen Armeniacae Amarum reference substance,the linear range of amygdalin was from 461 .7 to 1 5.39 mg/L (r =0.999 5),and the average recovery was 1 01 .1% (RSD =2.1 5%), The method was accurate,reliable,strong specialization and good repeatability.Conclusion:Semen Armeniacae Amarum reference substance can replace Semen Armeniacae Amarum reference drug for qualitative identification.