生物加工过程
生物加工過程
생물가공과정
CHINESE JOURNAL OF BIOPROCESS ENGINEERING
2015年
1期
216-219
,共4页
糖尿病肾病%早期%尿白蛋白%自拟益肾汤%PI3 K/AKT信号通路%细胞凋亡
糖尿病腎病%早期%尿白蛋白%自擬益腎湯%PI3 K/AKT信號通路%細胞凋亡
당뇨병신병%조기%뇨백단백%자의익신탕%PI3 K/AKT신호통로%세포조망
Diabetic Nephropathy%Early stage%Urinary albumin%Self-made kidney tonifying decoction%PI3K/AKT signaling pathway%Cell apoptosis
目的：观察自拟益肾汤对早期糖尿病肾病尿白蛋白的临床疗效及部分作用机制。方法：将本院收治的60例2型糖尿病肾病患者根据随机方法分为对照组（30例）和观察组（30例），2组患者均接受健康教育、合理饮食指导，内科降糖治疗，常规口服缬沙坦，80 mg／d；合并高脂血症者加服辛伐他汀，20 mg／d。观察组在上述常规治疗基础上加用自拟益肾汤，水煎药方200 mL，1剂／d（早晚分服），连用12周，1个疗程结束后观察2组患者治疗前后24 h尿微量白蛋白定量、糖化血红蛋白（HbAlc）、空腹血糖（FGC）、餐后2 h血糖（2 h BG）的变化；以及血清中 PI3K、Akt、p-Akt、bad、p-bad、Bcl-2，Bax、Caspase-3蛋白表达水平的变化。结果：1）2组治疗后24 h尿微量白蛋白定量、FPG、OGTT均较治疗前降低，与对照组比较，观察组降低的程度较为明显（P＜0．05）；2）2组治疗后血清中PI3K、p-Akt、p-bad、Bcl-2的蛋白表达均较治疗前升高， Bax、Caspase-3蛋白表达较治疗前降低，与对照组比较，观察组改变的幅度比较明显（P＜0．05）。结论：自拟益肾汤具有明显地降低糖尿病肾病蛋白尿作用，其机制可能与PI3 K／Akt信号转导通路有关。
目的：觀察自擬益腎湯對早期糖尿病腎病尿白蛋白的臨床療效及部分作用機製。方法：將本院收治的60例2型糖尿病腎病患者根據隨機方法分為對照組（30例）和觀察組（30例），2組患者均接受健康教育、閤理飲食指導，內科降糖治療，常規口服纈沙坦，80 mg／d；閤併高脂血癥者加服辛伐他汀，20 mg／d。觀察組在上述常規治療基礎上加用自擬益腎湯，水煎藥方200 mL，1劑／d（早晚分服），連用12週，1箇療程結束後觀察2組患者治療前後24 h尿微量白蛋白定量、糖化血紅蛋白（HbAlc）、空腹血糖（FGC）、餐後2 h血糖（2 h BG）的變化；以及血清中 PI3K、Akt、p-Akt、bad、p-bad、Bcl-2，Bax、Caspase-3蛋白錶達水平的變化。結果：1）2組治療後24 h尿微量白蛋白定量、FPG、OGTT均較治療前降低，與對照組比較，觀察組降低的程度較為明顯（P＜0．05）；2）2組治療後血清中PI3K、p-Akt、p-bad、Bcl-2的蛋白錶達均較治療前升高， Bax、Caspase-3蛋白錶達較治療前降低，與對照組比較，觀察組改變的幅度比較明顯（P＜0．05）。結論：自擬益腎湯具有明顯地降低糖尿病腎病蛋白尿作用，其機製可能與PI3 K／Akt信號轉導通路有關。
목적：관찰자의익신탕대조기당뇨병신병뇨백단백적림상료효급부분작용궤제。방법：장본원수치적60례2형당뇨병신병환자근거수궤방법분위대조조（30례）화관찰조（30례），2조환자균접수건강교육、합리음식지도，내과강당치료，상규구복힐사탄，80 mg／d；합병고지혈증자가복신벌타정，20 mg／d。관찰조재상술상규치료기출상가용자의익신탕，수전약방200 mL，1제／d（조만분복），련용12주，1개료정결속후관찰2조환자치료전후24 h뇨미량백단백정량、당화혈홍단백（HbAlc）、공복혈당（FGC）、찬후2 h혈당（2 h BG）적변화；이급혈청중 PI3K、Akt、p-Akt、bad、p-bad、Bcl-2，Bax、Caspase-3단백표체수평적변화。결과：1）2조치료후24 h뇨미량백단백정량、FPG、OGTT균교치료전강저，여대조조비교，관찰조강저적정도교위명현（P＜0．05）；2）2조치료후혈청중PI3K、p-Akt、p-bad、Bcl-2적단백표체균교치료전승고， Bax、Caspase-3단백표체교치료전강저，여대조조비교，관찰조개변적폭도비교명현（P＜0．05）。결론：자의익신탕구유명현지강저당뇨병신병단백뇨작용，기궤제가능여PI3 K／Akt신호전도통로유관。
Objective:To observe clinical effects of self-made kidney tonifying decoction on urinary albumin in patients with early Diabetic Nephropathy and to explore its partial mechanism.Methods:Sixty patients with type 2 diabetic nephropathy admitted at our hospital were randomly divided into control group (n=30)and observation group (n=30),and patients in both groups were given health education,diet instruction,internal medicine hypoglycemic therapy,80 mg/day of conventional oral valsartan.Those who also had hyperlipidemia were given extra treatment of simvastatin,20 mg/day.Observation group also applied the self-made kidney tonifying decoction based on the conventional treatment,200 ml water tisanes,1 agent daily in the morning and evening,for 1 2 continuous weeks.After a period of treatment,24h microal buminuria quantitative determination,glycosylated hemoglobin (HbAlc),fasting blood-glucose (FGC),2 h postprandial blood glucose (2 HBG)changes of patients of the two groups before and after treatment were recorded;and protein expression level changes of serum PI3K,Akt,p - Akt,bad,p - bad,Bcl 2,Bax and Caspase 3 were measured.Results:1 )The levels of 24h microalbuminuria quantitative determination,FPG,OGTT after treatment were reduced compared to those before treatment in both groups,and the reduction extent of observation group were more obvious compared with those of the control group (P<0. 05);2)the levels of serum PI3K,p-Akt,p-bad,the Bcl-2 protein expression in the two groups after treatment were increased compared to those before treatment,and the Bax,Caspase 3 protein expression was re-duced,the change magnitude of observation group were more obvious compared with those of the control group (P<0. 05 ).Con-clusion:Self-made kidney tonifying decoction has obvious effect to reduce proteinuria of diabetic nephropathy,and its mechanism may be associated with PI3K/Akt signal transduction pathway.
