中国心血管杂志
中國心血管雜誌
중국심혈관잡지
CHINESE JOURNAL OF CARDIOVASOLOGY
2015年
1期
52-56
,共5页
李永东%葛智平%温慧华%刘丹%阿拉坦高勒
李永東%葛智平%溫慧華%劉丹%阿拉坦高勒
리영동%갈지평%온혜화%류단%아랍탄고륵
高血压%内皮祖细胞%凋亡%氧化应激%拮抗剂AMD 3100
高血壓%內皮祖細胞%凋亡%氧化應激%拮抗劑AMD 3100
고혈압%내피조세포%조망%양화응격%길항제AMD 3100
Hypertension%Endothelial progenitor cells%Apoptosis%Oxidative stress%Antagonist AMD 3100
目的：探讨贝那普利对高血压患者内皮祖细胞( EPC）凋亡和氧化应激的影响。方法入选符合《中国高血压防治指南》诊断标准的1级高血压患者15例,同时选取年龄、性别相匹配的健康体检者15名为对照组。所有受试者抽取外周血,常规密度梯度离心法分离单个核细胞,将经鉴定的正在分化的EPC培养5 d后用于实验。将分离、培养获得的每一例高血压患者EPC培养5 d后将细胞平行分为5份,每份细胞根据不同的刺激处理条件分为高血压组、不同浓度贝那普利(1、10和100 umol/L）干预组、贝那普利(10 umol/L）+基质细胞衍生因子/受体趋化因子4(SDF-1/CXCR4）拮抗剂AMD3100干预组。上述干预24 h后进行EPC凋亡检测：用Annexin-V/PI法；氧化应激检测：黄嘌呤氧化法测定上清液中超氧化物歧化酶活力,硫代巴比妥酸法测定丙二醛含量。结果(1）与对照组比较,高血压组外周血EPC凋亡率明显增加(t早=6.6334,t晚=3.812；均为p<0.01）,氧化应激反应明显增高(tSOD =8.258,tMDA =6.6806；均为p<0.01）；(2）随着贝那普利干预浓度的增加(1、10和100 umol/L）,EPC凋亡率也随之降低(F早=17.39,F晚=51.65；均为p<0.05）,氧化应激反应也随之降低,呈浓度依赖性(FSOD =14.56,FMDA =7.859；均为p<0.05）。与10 umol/L贝那普利干预组比较,10 umol/L贝那普利+AMD 3100干预组的EPC凋亡率增加(t早=3.551,t晚=5.333；均为p早、晚<0.05）,氧化应激反应增高(tSOD =1.931,tMDA =0.6407；均为p<0.05）。结论贝那普利能显著改善高血压患者体外EPC凋亡及氧化应激反应,并可能通过SDF-1/CXCR4轴发挥作用。
目的：探討貝那普利對高血壓患者內皮祖細胞( EPC）凋亡和氧化應激的影響。方法入選符閤《中國高血壓防治指南》診斷標準的1級高血壓患者15例,同時選取年齡、性彆相匹配的健康體檢者15名為對照組。所有受試者抽取外週血,常規密度梯度離心法分離單箇覈細胞,將經鑒定的正在分化的EPC培養5 d後用于實驗。將分離、培養穫得的每一例高血壓患者EPC培養5 d後將細胞平行分為5份,每份細胞根據不同的刺激處理條件分為高血壓組、不同濃度貝那普利(1、10和100 umol/L）榦預組、貝那普利(10 umol/L）+基質細胞衍生因子/受體趨化因子4(SDF-1/CXCR4）拮抗劑AMD3100榦預組。上述榦預24 h後進行EPC凋亡檢測：用Annexin-V/PI法；氧化應激檢測：黃嘌呤氧化法測定上清液中超氧化物歧化酶活力,硫代巴比妥痠法測定丙二醛含量。結果(1）與對照組比較,高血壓組外週血EPC凋亡率明顯增加(t早=6.6334,t晚=3.812；均為p<0.01）,氧化應激反應明顯增高(tSOD =8.258,tMDA =6.6806；均為p<0.01）；(2）隨著貝那普利榦預濃度的增加(1、10和100 umol/L）,EPC凋亡率也隨之降低(F早=17.39,F晚=51.65；均為p<0.05）,氧化應激反應也隨之降低,呈濃度依賴性(FSOD =14.56,FMDA =7.859；均為p<0.05）。與10 umol/L貝那普利榦預組比較,10 umol/L貝那普利+AMD 3100榦預組的EPC凋亡率增加(t早=3.551,t晚=5.333；均為p早、晚<0.05）,氧化應激反應增高(tSOD =1.931,tMDA =0.6407；均為p<0.05）。結論貝那普利能顯著改善高血壓患者體外EPC凋亡及氧化應激反應,併可能通過SDF-1/CXCR4軸髮揮作用。
목적：탐토패나보리대고혈압환자내피조세포( EPC）조망화양화응격적영향。방법입선부합《중국고혈압방치지남》진단표준적1급고혈압환자15례,동시선취년령、성별상필배적건강체검자15명위대조조。소유수시자추취외주혈,상규밀도제도리심법분리단개핵세포,장경감정적정재분화적EPC배양5 d후용우실험。장분리、배양획득적매일례고혈압환자EPC배양5 d후장세포평행분위5빈,매빈세포근거불동적자격처리조건분위고혈압조、불동농도패나보리(1、10화100 umol/L）간예조、패나보리(10 umol/L）+기질세포연생인자/수체추화인자4(SDF-1/CXCR4）길항제AMD3100간예조。상술간예24 h후진행EPC조망검측：용Annexin-V/PI법；양화응격검측：황표령양화법측정상청액중초양화물기화매활력,류대파비타산법측정병이철함량。결과(1）여대조조비교,고혈압조외주혈EPC조망솔명현증가(t조=6.6334,t만=3.812；균위p<0.01）,양화응격반응명현증고(tSOD =8.258,tMDA =6.6806；균위p<0.01）；(2）수착패나보리간예농도적증가(1、10화100 umol/L）,EPC조망솔야수지강저(F조=17.39,F만=51.65；균위p<0.05）,양화응격반응야수지강저,정농도의뢰성(FSOD =14.56,FMDA =7.859；균위p<0.05）。여10 umol/L패나보리간예조비교,10 umol/L패나보리+AMD 3100간예조적EPC조망솔증가(t조=3.551,t만=5.333；균위p조、만<0.05）,양화응격반응증고(tSOD =1.931,tMDA =0.6407；균위p<0.05）。결론패나보리능현저개선고혈압환자체외EPC조망급양화응격반응,병가능통과SDF-1/CXCR4축발휘작용。
Objective To investigate the effection of Benner Pury on endothelial progenitor cells apoptosis and oxidative stress in vitro hypertension. Methods The patients who were diagnosed as hypertension grade 1, according to the standard ″Guidelines for Prevention and Treatment of Hypertension″. The control group were healthy subjects matched with study group in age and gender. Mononuclear cells were isolated by density gradient centrifugation, and fluorescence chemical staining in acLDL-DiI, FITC-lectin, observed under confocal laser scanning microscope, staining positive cells were considered as the differentiation of endothelial progenitor cell(EPC). EPC cultivated for 5 days were used for study. The cells isolated from each hypertensive were divided into five copies in parallel, and according to the different stimulation, the cells were divided into five groups: hypertension group, different concentrations of Benner Pury (1 umol/L, 10 umol/L, 100 umol/L) intervention in hypertension group, and Benner Pury (10 umol/L) +stromal cell derived factor-1(SDF-1)/its receptor chemokine 4 (CXCR4) antagonist AMD 3100 pretreatment group. EPC cultivated for 24 h under the different stimulation were used for study EPC apoptosis detection:Annexin-V/PI method; oxidative stress detection: the activity of superoxide dismutase ( SOD) , xanthine-oxidation method, the content of Malonaldehyde ( MDA) malondialdehyde thiobarbituric acid method. Results ( 1 ) Compared with the control group, peripheral blood EPS apoptosis rate was obviously increased in hypertension group, oxidative stress was significantly increased too ( tearly =6. 6334, tlate =3. 812; both p<0. 01). (2) Detection of the apoptosis and oxidative stress level display, different concentrations of Benner Pury 1 umol/L, 10 umol/L, 100 umol/L intervention in vitro hypertensive patients EPC for 24 h, with the concentrations of Benner Pury gradually increased, the apoptosis rate of EPC was reduced (Fearly =17. 39, Flate =51. 65;both p<0. 05), oxidative stress response also come down in a concentration dependent manner (FSOD =14. 56, FMDA =7. 859;both p<0. 05). Compared with 10 umol/L pretreatment group, 10 umol/L+group AMD 3100, EPC apoptosis rate increased ( tearly =3. 551, tlate =5. 333, both p<0. 05), oxidative stress response raised up (tSOD =1. 931, tMDA =0. 6407, both p<0. 05). Conclusions BennerPury significantly improved the apoptosis of EPC and oxidative stress response in vitro hypertension, and the function may be through the SDF-1/CXCR4 axis.
