CAJ | 학술논문

透射电镜技术中前期样品制备是获得最接近样品真实结构信息的关键。本文选择酵母为观察对象，运用常规样品制备技术，观察不同缓冲液、不同脱水时间和脱水温度对酵母超微结构的影响，优化了酵母常规透射电镜前期制样方案。
투사전경기술중전기양품제비시획득최접근양품진실결구신식적관건。본문선택효모위관찰대상，운용상규양품제비기술，관찰불동완충액、불동탈수시간화탈수온도대효모초미결구적영향，우화료효모상규투사전경전기제양방안。
Sample preparation for transmission electron microscopy is the most important step to preserve the biological ultrastructure. In this study, yeast cells were treated with different buffers, and dehydrated in different time periods at different temperatures. By morphological comparison, we got an optimized strategy for conventional method for yeast cell sample preparation for transmission electron microscopy.