CAJ | 학술논문

扫描电镜是观察植物表皮蜡质结构的极佳方法。由于蜡质成分不同，不同的植物有不同的样品干燥方法。本文以拟南芥叶片为实验材料，比较FAA固定和戊二醛－锇酸双固定，CO2临界点干燥和冷冻干燥，以及乙醇、乙酸异戊酯、丙酮和叔丁醇等不同的固定方法、不同的干燥方法和不同的有机溶剂对蜡质的溶解作用后，认为更适于拟南芥表皮蜡质观察的样品制备方法是：戊二醛－锇酸双固定，叔丁醇与乙醇梯度脱水，叔丁醇冷冻干燥。
소묘전경시관찰식물표피사질결구적겁가방법。유우사질성분불동，불동적식물유불동적양품간조방법。본문이의남개협편위실험재료，비교FAA고정화무이철－철산쌍고정，CO2림계점간조화냉동간조，이급을순、을산이무지、병동화숙정순등불동적고정방법、불동적간조방법화불동적유궤용제대사질적용해작용후，인위경괄우의남개표피사질관찰적양품제비방법시：무이철－철산쌍고정，숙정순여을순제도탈수，숙정순냉동간조。
Waxy layer of the plant epidermis is the first barrier to resistant environmental stimulation. Scanning electron microscopy ( SEM) is a useful technique to observe the leaf epicuticular waxes of Arabidopsis. Different sample preparation methods for SEM observation were compared including critical point drying and freeze drying, FAA fixation and glutaraldehyde?osmium tetroxide double fixation. The effects of different organic solvents on wax structure were also studied. The optimal method to examine epicuticular waxes contains the following processes:glutaraldehyde?osmium tetroxide double fixation, dehydration of tert?butanol and ethanol gradient, and freeze drying.