CAJ | 학술논문

本实验利用胶体金免疫电镜技术检测番茄环纹斑点病毒（ tomato zonate spot virus， TZSV），分别以TZSV的N 蛋白多克隆抗体及TZSV的Gn蛋白多克隆抗体为一抗，然后以胶体金标记羊抗兔IgG?gold（10 nm）为二抗对病毒进行胶体金标记，电镜观察用TZSV Gn蛋白抗体标记的胶体金颗粒能很好地结合到病毒粒体上。结果表明该方法可以实现对TZSV更为准确可靠地检测。
본실험이용효체금면역전경기술검측번가배문반점병독（ tomato zonate spot virus， TZSV），분별이TZSV적N 단백다극륭항체급TZSV적Gn단백다극륭항체위일항，연후이효체금표기양항토IgG?gold（10 nm）위이항대병독진행효체금표기，전경관찰용TZSV Gn단백항체표기적효체금과립능흔호지결합도병독립체상。결과표명해방법가이실현대TZSV경위준학가고지검측。
In this study, the immunogold labeling electron microscopy was used to detect Tomato zonate spot virus (TZSV). The polyclonal antibody against TZSV N protein and the polyclonal antibody against TZSV Gn protein were used as primary antibody, respectively, and the colloidal gold labeleld Goat anti?rabbit IgG?gold (10 nm) was used as the secondary antibody. Under the electron microscopy, TZSV particles labelled with colloidal gold particles could be observed when the polyclonal antibody against TZSV Gn protein was used as primary antibody. The result indicated that it was an accurate and reliable method to detect TZSV.