CAJ | 학술논문

目的：研究走马胎中化合物AG4对人鼻咽癌细胞裸鼠移植瘤生长的影响。方法：建立人鼻咽癌细胞裸鼠皮下移植瘤模型，以抑瘤率为指标研究化合物AG4的体内抑瘤作用及AG4干预对裸鼠的一般情况（体质量、活动、食欲等）的影响；同时采用实时荧光定量PCR法观察AG4对肿瘤内凋亡相关基因表达的影响。结果：阳性药顺铂组（2.00 mg·kg-1）对荷瘤裸鼠的抑瘤率为49.41%，3.02 mg·kg-1AG4组的抑瘤率为42.34%，AG4对裸鼠体质量及肝、脾、肾的脏器指数没有明显影响；AG4能明显升高Bax和Bad基因的相对表达量，降低Bcl-2基因的相对表达量。结论：AG4在裸鼠体内对人鼻咽癌细胞有较强的抑制作用，对裸鼠没有明显的毒副作用，其作用机制可能与激活线粒体途径诱导肿瘤细胞凋亡有关。
목적：연구주마태중화합물AG4대인비인암세포라서이식류생장적영향。방법：건립인비인암세포라서피하이식류모형，이억류솔위지표연구화합물AG4적체내억류작용급AG4간예대라서적일반정황（체질량、활동、식욕등）적영향；동시채용실시형광정량PCR법관찰AG4대종류내조망상관기인표체적영향。결과：양성약순박조（2.00 mg·kg-1）대하류라서적억류솔위49.41%，3.02 mg·kg-1AG4조적억류솔위42.34%，AG4대라서체질량급간、비、신적장기지수몰유명현영향；AG4능명현승고Bax화Bad기인적상대표체량，강저Bcl-2기인적상대표체량。결론：AG4재라서체내대인비인암세포유교강적억제작용，대라서몰유명현적독부작용，기작용궤제가능여격활선립체도경유도종류세포조망유관。
Objective:To investigate the influence of compound AG4 derived fromArdisia gigantifolia Stapf. on xenograft tumor of human nasopharyngeal carcinoma (NPC) in nude mice.Methods: Human NPC nude mice model were established, and the inhibition rate (IR) was the index to evaluate the tumor growth suppression of AG4in vivo. Simultaneously, general conditions (weight, action, appetite) of nude mice and the expression of Bcl-2, Bad and Bax mRNA by real time PCR method were observed during AG4 interference.Results: The IR of positive control group (cisplatin 2.00 mg·kg-1) and AG4 group (3.02 mg·kg-1) was 49.41% and 42.34% respectively. The effect of AG4 on the weight lose of nude mice was not significant comparing with negative control group, and the toxicity of AG4 to the vital organs (liver, spleen and kidney) and immunity index (spleen index) was not obvious in short period. AG4 could increase the Bax and Bad mRNA expression, and decrease the Bcl-2 mRNA expression.Conclusion:AG4 had obvious inhibition effects on xenograft tumor in human NPC nude mice, and with no outstanding adverse effects, the mechanisms may relate to the induction of tumor apoptosis due to the activation of mitochondria signal pathway.