CAJ | 학술논문

目的：建立齿囊耙齿菌固体发酵产物中麦角甾醇含量的测定方法。方法采用高效液相色谱方法，汉邦ODS-C18色谱分析柱（250×4.6mm，5μm），流动相为100％甲醇，流速1.0mL/min，检测波长282nm；柱温30℃。结果麦角甾醇进样量在0.20～1.40μg之间与吸收峰面积呈良好的线性关系，r=0.9999；平均加样回收率为100.07%，RSD（%）=1.91%。方法具有较高的专属性、准确性、重现性和可行性。结论该方法简便、准确、灵敏度好，为齿囊耙齿菌固体发酵产物质量评价提供了可靠依据。
목적：건립치낭파치균고체발효산물중맥각치순함량적측정방법。방법채용고효액상색보방법，한방ODS-C18색보분석주（250×4.6mm，5μm），류동상위100％갑순，류속1.0mL/min，검측파장282nm；주온30℃。결과맥각치순진양량재0.20～1.40μg지간여흡수봉면적정량호적선성관계，r=0.9999；평균가양회수솔위100.07%，RSD（%）=1.91%。방법구유교고적전속성、준학성、중현성화가행성。결론해방법간편、준학、령민도호，위치낭파치균고체발효산물질량평개제공료가고의거。
Objective To establish a method for determination of ergosterol content in irpex lacteus sac fungi solid fermentation products. Methods Using high performance liquid chromatography, Hanbond ODS-C18 column chromatography (250×4.6mm, 5μm), the mobile phase of 100% methanol, flow rate was 1.0mL/ min, detection wavelength was 282nm;column temperature was 30℃. Results The injection volume of ergosterol was between 0.20-1.40μg absorption peak area, showed a good linear relationship, r=0.9999; the average recovery was 100.07%, RSD(%)=1.91%. The method had a high specificity, accuracy, reproducibility and feasibility. Conclusion The method is simple, accurate, sensitive, good, it provides a reliable basis for quality evaluation of irpex lacteus sac fungi solid fermentation product.