粮食与油脂
糧食與油脂
양식여유지
CEREALS & OILS
2015年
2期
59-62
,共4页
宽叶独行菜%超声辅助%多糖%响应面
寬葉獨行菜%超聲輔助%多糖%響應麵
관협독행채%초성보조%다당%향응면
Lepidium latifolium%ultrasonic-assisted%polysaccharide%response surface methodology
为优化宽叶独行菜多糖的提取工艺，在单因素实验的基础上，选取超声功率、粒度、液料比和提取时间作为优化因素，根据Box–Behnken实验设计原理，进行四因素三水平实验。利用响应面分析方法，建立了宽叶独行菜多糖提取得率的多元二次回归方程，并得到最佳提取工艺条件。结果表明，提取时间对宽叶独行菜多糖提取得率的影响最为显著。当工艺条件为功率124 W、粒度120目、液料比24∶1 mL/g、提取时间30 min时，宽叶独行菜多糖的理论最高提取得率为0.315%，验证值为0.319%。
為優化寬葉獨行菜多糖的提取工藝，在單因素實驗的基礎上，選取超聲功率、粒度、液料比和提取時間作為優化因素，根據Box–Behnken實驗設計原理，進行四因素三水平實驗。利用響應麵分析方法，建立瞭寬葉獨行菜多糖提取得率的多元二次迴歸方程，併得到最佳提取工藝條件。結果錶明，提取時間對寬葉獨行菜多糖提取得率的影響最為顯著。噹工藝條件為功率124 W、粒度120目、液料比24∶1 mL/g、提取時間30 min時，寬葉獨行菜多糖的理論最高提取得率為0.315%，驗證值為0.319%。
위우화관협독행채다당적제취공예，재단인소실험적기출상，선취초성공솔、립도、액료비화제취시간작위우화인소，근거Box–Behnken실험설계원리，진행사인소삼수평실험。이용향응면분석방법，건립료관협독행채다당제취득솔적다원이차회귀방정，병득도최가제취공예조건。결과표명，제취시간대관협독행채다당제취득솔적영향최위현저。당공예조건위공솔124 W、립도120목、액료비24∶1 mL/g、제취시간30 min시，관협독행채다당적이론최고제취득솔위0.315%，험증치위0.319%。
The extraction technologyof polysaccharide from Lepidium latifolium wasoptimized with the extraction yield as the assessment index. On the basisof single–factor experiments,four factors including ultrasonic power,material mesh,liquid–to–solid ratio and extraction time were selected as the independent variables. With response surface analysis,a quadratic regression model was established by the Box–Behnken design experiments. The results showed that extraction time was the most significant factor to polysaccharide yield. Theoptimum extractionconditions were as follows:ultrasonic power 124 W,material mesh 120,liquid–to–solid ratio 24∶1 mL /g,and extraction time 30 min. Under such conditions,the predicted and experimental valuesof polysaccharide yield from Lepidium latifolium were 0.315% and 0.319%,respectively.
