CAJ | 학술논문

利用碱性蛋白酶Alcalase 2.4L水解大豆分离蛋白，研究了底物浓度、加酶量、pH、温度因素对酶解液水解度和还原力的影响，并在此基础上用响应面分析法优化酶水解条件。以还原力为主要指标考察酶解产物的抗氧化活性，通过水解度与还原力的比较，得到Alcalase 2.4L碱性蛋白酶酶解大豆分离蛋白产物的抗氧化活性与水解度之间没有直接线性关系。在实验得到的最佳条件下制备的大豆肽抗氧化活性良好，具有良好的应用前景。
이용감성단백매Alcalase 2.4L수해대두분리단백，연구료저물농도、가매량、pH、온도인소대매해액수해도화환원력적영향，병재차기출상용향응면분석법우화매수해조건。이환원력위주요지표고찰매해산물적항양화활성，통과수해도여환원력적비교，득도Alcalase 2.4L감성단백매매해대두분리단백산물적항양화활성여수해도지간몰유직접선성관계。재실험득도적최가조건하제비적대두태항양화활성량호，구유량호적응용전경。
Alkaline protease alcalase 2.4 L was used to hydrolyze soy protein isolate,the substrate concentration,the amountof enzyme,pH and hydrolysis temperatureon the degreeof hydrolysis and the reducing powerof enzymatic hydrolysates were studied,and the enzymatic hydrolysatesconditions wereoptimized by using response surface methodology. There was no direct linearcorrelation between antioxidant activity and degreeof hydrolysis by taking the reducing power as the main indicator. Under optimalconditions,antioxidant activityof soy peptides had excellent antioxidant which had good prospect.