CAJ | 학술논문

目的：探讨巨噬细胞移动抑制因子（MIF）、CD74、血管内皮生长因子（VEGF）在膀胱尿路上皮癌组织中的表达及其临床意义。方法采用免疫组织化学方法对80例膀胱尿路上皮癌患者癌组织和15例膀胱正常组织中MIF、CD74和VEGF的蛋白表达水平进行检测，并分析其与临床病理特征和预后的关系。结果 MIF、CD74和VEGF在膀胱尿路上皮癌组织中高表达，在正常膀胱黏膜组织中无或弱表达，差异有统计学意义（P＜0．05）。肿瘤细胞胞质内MIF、CD74和VEGF的表达与组织学分类、病理分级、临床分期均呈正相关（P＜0．05）。而且相关性分析发现，MIF与CD74在膀胱尿路上皮癌组织中的表达呈正相关（r＝0．839，P＜0．05），且MIF与VEGF在膀胱尿路上皮癌组织中的表达亦呈正相关（r＝0．753，P＜0．05）。结论 MIF可能通过与CD74相互作用，上调VEGF表达促进肿瘤微血管生成，促进膀胱尿路上皮癌的发生发展，使其有望成为膀胱尿路上皮癌治疗的有效靶分子。
목적：탐토거서세포이동억제인자（MIF）、CD74、혈관내피생장인자（VEGF）재방광뇨로상피암조직중적표체급기림상의의。방법채용면역조직화학방법대80례방광뇨로상피암환자암조직화15례방광정상조직중MIF、CD74화VEGF적단백표체수평진행검측，병분석기여림상병리특정화예후적관계。결과 MIF、CD74화VEGF재방광뇨로상피암조직중고표체，재정상방광점막조직중무혹약표체，차이유통계학의의（P＜0．05）。종류세포포질내MIF、CD74화VEGF적표체여조직학분류、병리분급、림상분기균정정상관（P＜0．05）。이차상관성분석발현，MIF여CD74재방광뇨로상피암조직중적표체정정상관（r＝0．839，P＜0．05），차MIF여VEGF재방광뇨로상피암조직중적표체역정정상관（r＝0．753，P＜0．05）。결론 MIF가능통과여CD74상호작용，상조VEGF표체촉진종류미혈관생성，촉진방광뇨로상피암적발생발전，사기유망성위방광뇨로상피암치료적유효파분자。
Objective To investigate the expressions and clinical significance of macrophage migration inhibi‐tory factor (MIF) ,CD74 and vascular endothelial growth factor (VEGF) in the urothelial cell carcinoma of bladder . Methods The expressions of MIF ,CD74 and VEGF in 80 samples of urothelial cell carcinoma bladder tissues and 15 samples of normal bladder tissues were detected by immunohistochemistry method ,and the relationships between these indicators with clinicopathological features and prognosis were analyzed .Results The expressions of MIF , CD74 and VEGF in the bladder urothelial cell carcinoma tissues elevated significantly compared with those of the nor‐mal bladder tissues ,the differences were significant (P< 0 .05) .The expressions of MIF ,CD74 and VEGF in the tumor cell plasma were positively related with the histological classification ,pathological grade and clinical stages (P<0 .05) .The correlation analysis showed that the expression of MIF was positively related with CD 74 and VEGF in the bladder urothelial cell carcinoma tissues (r=0 .839;r=0 .753 ,P<0 .05) .Conclusion MIF might interact with CD74 to upregulate the expression of VEGF ,promote the angiogenesis and involved in the tumor development and progression ,MIF might be an effective molecular target in the treatment of urothelial cell carcinoma of the urinary bladder .