检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2015年
4期
454-456,459
,共4页
肠道腺癌%多药耐药%免疫组织化学
腸道腺癌%多藥耐藥%免疫組織化學
장도선암%다약내약%면역조직화학
colorectal carcinoma%multidrug resistance gene%immunohisto-chemistry
目的：探讨肠道腺癌中肺耐药相关蛋白（LRP）、P‐糖蛋白（P‐gp／P170）、拓扑易构酶（ToPoⅡ）、胸腺嘧啶合成酶（Ts）及谷胱甘肽转移酶（GST ）‐π的表达水平及其临床意义。方法采用免疫组化法检测96例原发性肠道腺癌及15例正常肠黏膜中Ts、P170、ToPoⅡ、LRP、GST‐π的表达情况，并综合分析其与各临床参数和预后的关系。结果 LRP、P170、ToPoⅡ、Ts在肠道腺癌组织中呈高表达状态；5种蛋白在癌组织中的表达水平与年龄、性别无关，但在有淋巴结转移者表达高于无淋巴结转移者，LRP、P170在低分化腺癌组织中的表达明显高于高中分化腺癌，而Ts的表达明显低于高中分化腺癌；浸润肌层及以内的腺癌表达 ToPoⅡ明显高于浸润至浆膜者；LRP、P170、Ts阳性表达患者的5年存活率低于阴性表达者。结论 Ts、P170、ToPoⅡ、LRP、GST‐π5种耐药蛋白与肠道腺癌分化程度、浸润深度、淋巴结转移以及5年存活率均有不同程度的相关性，这在制订肠道腺癌的个性化化疗方案以及患者预后评估上具有重要的临床意义。
目的：探討腸道腺癌中肺耐藥相關蛋白（LRP）、P‐糖蛋白（P‐gp／P170）、拓撲易構酶（ToPoⅡ）、胸腺嘧啶閤成酶（Ts）及穀胱甘肽轉移酶（GST ）‐π的錶達水平及其臨床意義。方法採用免疫組化法檢測96例原髮性腸道腺癌及15例正常腸黏膜中Ts、P170、ToPoⅡ、LRP、GST‐π的錶達情況，併綜閤分析其與各臨床參數和預後的關繫。結果 LRP、P170、ToPoⅡ、Ts在腸道腺癌組織中呈高錶達狀態；5種蛋白在癌組織中的錶達水平與年齡、性彆無關，但在有淋巴結轉移者錶達高于無淋巴結轉移者，LRP、P170在低分化腺癌組織中的錶達明顯高于高中分化腺癌，而Ts的錶達明顯低于高中分化腺癌；浸潤肌層及以內的腺癌錶達 ToPoⅡ明顯高于浸潤至漿膜者；LRP、P170、Ts暘性錶達患者的5年存活率低于陰性錶達者。結論 Ts、P170、ToPoⅡ、LRP、GST‐π5種耐藥蛋白與腸道腺癌分化程度、浸潤深度、淋巴結轉移以及5年存活率均有不同程度的相關性，這在製訂腸道腺癌的箇性化化療方案以及患者預後評估上具有重要的臨床意義。
목적：탐토장도선암중폐내약상관단백（LRP）、P‐당단백（P‐gp／P170）、탁복역구매（ToPoⅡ）、흉선밀정합성매（Ts）급곡광감태전이매（GST ）‐π적표체수평급기림상의의。방법채용면역조화법검측96례원발성장도선암급15례정상장점막중Ts、P170、ToPoⅡ、LRP、GST‐π적표체정황，병종합분석기여각림상삼수화예후적관계。결과 LRP、P170、ToPoⅡ、Ts재장도선암조직중정고표체상태；5충단백재암조직중적표체수평여년령、성별무관，단재유림파결전이자표체고우무림파결전이자，LRP、P170재저분화선암조직중적표체명현고우고중분화선암，이Ts적표체명현저우고중분화선암；침윤기층급이내적선암표체 ToPoⅡ명현고우침윤지장막자；LRP、P170、Ts양성표체환자적5년존활솔저우음성표체자。결론 Ts、P170、ToPoⅡ、LRP、GST‐π5충내약단백여장도선암분화정도、침윤심도、림파결전이이급5년존활솔균유불동정도적상관성，저재제정장도선암적개성화화료방안이급환자예후평고상구유중요적림상의의。
Objective To investigate the expression and clinical significance of Thymine synthetase (Ts) ,P‐glycoprotein(P170) ,toposiomerase(ToPoⅡ) ,lung resistance‐related protein(LRP) ,glu tathione tfan sferase(GST‐π) in colorectal carcinoma tissue .Methods The expression of Ts ,P170 ,ToPoⅡ ,LRP and GST‐πin 96 patients with colorectal carcinoma and 15 healthy persons were detected by immunohistochemical technique ,and their relationship with clinical indicators and prognosis of carcinoma were explored .Results LRP ,P170 ,ToPo Ⅱ and Ts in in testinal tissues showed high expression in intestinal adenocarcinoma .The five kinds proteins expression levels in the adeno‐carcinoma had no relationship with age and gender ,but these were higher in the cases with lymph node metastasis than those without lymph node metastasis .The levels of LRP ,P170 in low differentiation adenocarcinoma were sig‐nificant higher than that in high differentiation adenocarcinoma ,while the expression of Ts was significant lower than that in high differentiation adenocarcinoma .The expression of ToPo Ⅱ was significant higher in invasion muscle layer than that of infiltrated serosa .The five years survival rate of patients with positive expression of LRP ,P170 were lower than that of the negative expression cases .Conclusion Ts ,P170 ,ToPo Ⅱ ,LRP and GST‐πhave relationships with intestinal adenocarcinoma differentiation degree ,invasion depth ,lymph node metastasis and the survival rate of five years ,these relationships important for personalized chemotherapy scheme and prognostic evaluation .
