中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2015年
2期
261-264
,共4页
梗阻性黄疸%水通道蛋白%肾功损害
梗阻性黃疸%水通道蛋白%腎功損害
경조성황달%수통도단백%신공손해
Obstructive jaundice%Aquaporin-2%Renal injury
目的：明确水通道蛋白2（ AQP2）在梗阻性黄疸大鼠肾脏表达变化及其与尿素氮（ BUN）和肌酐（ Cr）变化的关系。方法：采用手术结扎胆总管的方法造成实验大鼠梗阻性黄疸模型，于术后3、5、7、10、14 d处死大鼠取血和肾脏标本，检测血浆直接胆红素、尿素氮和肌酐值，光镜下观察肾脏病理形态学，免疫组化方法检测肾脏APQ2表达。结果：实验组3 d时光镜下见肾集合管上皮细胞排列不规则肿胀，5 d时可见细胞水样空泡变性、并且梗阻性黄疸组AQP2表达较对照组明显减少。7 d时见部分细胞脱落、间质有炎性细胞浸润，10和14 d时见局灶上皮细胞坏死、胞浆崩解、间质有大量炎性细胞浸润。水通道蛋白2表达在梗阻5 d后较对照组明显减少（ P＜0.05），随着梗阻时间延长，其表达减少愈加明显。 AQP2表达减少的同时，肾形态学损害在光镜下从肾集合管形态学改变得到证实。梗阻性黄疸组术后10 d Cr开始升高，梗阻14 d后BUN开始升高。结论：AQP2的减少表明肾功能发生损害，AQP2减少在前，CR和BUN增加在后，所以AQP2作为肾功能损害的早期检测指标是很有意义的。
目的：明確水通道蛋白2（ AQP2）在梗阻性黃疸大鼠腎髒錶達變化及其與尿素氮（ BUN）和肌酐（ Cr）變化的關繫。方法：採用手術結扎膽總管的方法造成實驗大鼠梗阻性黃疸模型，于術後3、5、7、10、14 d處死大鼠取血和腎髒標本，檢測血漿直接膽紅素、尿素氮和肌酐值，光鏡下觀察腎髒病理形態學，免疫組化方法檢測腎髒APQ2錶達。結果：實驗組3 d時光鏡下見腎集閤管上皮細胞排列不規則腫脹，5 d時可見細胞水樣空泡變性、併且梗阻性黃疸組AQP2錶達較對照組明顯減少。7 d時見部分細胞脫落、間質有炎性細胞浸潤，10和14 d時見跼竈上皮細胞壞死、胞漿崩解、間質有大量炎性細胞浸潤。水通道蛋白2錶達在梗阻5 d後較對照組明顯減少（ P＜0.05），隨著梗阻時間延長，其錶達減少愈加明顯。 AQP2錶達減少的同時，腎形態學損害在光鏡下從腎集閤管形態學改變得到證實。梗阻性黃疸組術後10 d Cr開始升高，梗阻14 d後BUN開始升高。結論：AQP2的減少錶明腎功能髮生損害，AQP2減少在前，CR和BUN增加在後，所以AQP2作為腎功能損害的早期檢測指標是很有意義的。
목적：명학수통도단백2（ AQP2）재경조성황달대서신장표체변화급기여뇨소담（ BUN）화기항（ Cr）변화적관계。방법：채용수술결찰담총관적방법조성실험대서경조성황달모형，우술후3、5、7、10、14 d처사대서취혈화신장표본，검측혈장직접담홍소、뇨소담화기항치，광경하관찰신장병리형태학，면역조화방법검측신장APQ2표체。결과：실험조3 d시광경하견신집합관상피세포배렬불규칙종창，5 d시가견세포수양공포변성、병차경조성황달조AQP2표체교대조조명현감소。7 d시견부분세포탈락、간질유염성세포침윤，10화14 d시견국조상피세포배사、포장붕해、간질유대량염성세포침윤。수통도단백2표체재경조5 d후교대조조명현감소（ P＜0.05），수착경조시간연장，기표체감소유가명현。 AQP2표체감소적동시，신형태학손해재광경하종신집합관형태학개변득도증실。경조성황달조술후10 d Cr개시승고，경조14 d후BUN개시승고。결론：AQP2적감소표명신공능발생손해，AQP2감소재전，CR화BUN증가재후，소이AQP2작위신공능손해적조기검측지표시흔유의의적。
Objective:To investigate the change of expression of AQP 2 in renal of obstructive jaundice rats , and the relationship between AQP 2 and the changing of Cr and BUN.Methods: Legated the common bile duct of rats to form the obstructive jaundice group.Scarified the rats on the 3th,5th,7th,10th,14th day,took blood samples and the kidney of the rats.Test direct bilirubin BUN and Cr in serum,the renal histopathological changes were observed by optical microscopy .The expression of AQP2 in renal of rats was tested by using radio immunological method.Results: Light microscopic examination of kidney showed that swelling epithelium arranged irregularly in 3rd day ,bleb in the 5th day.The expression of AQP2 in renal of rats with obstructive jaundice were greatly less than sham operation.Mesenchyma inflammatory cells infiltrate in 7th day.Local epithelial necrosis and lots of inflammatory cells infiltrate in the mesenchyma in 10th day and 14th day.The expression of AQP2 in renal collective tubule was decreased on the 5th day compared with sham operation ,and the decrease was more as time gone by.The renal function injury can be confirmed though the renal collective tubule change.Cr and BUN in serum began to increase on the 10th day and 14th day.Conclusion: Decrease of AQP2 is earlier than the increase of Cr and BUN ,and it can be the early sign of renal function injury.
