中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2015年
2期
253-256
,共4页
李红林%高美华%郑云会%杨士军%刘艳秋%陆卫平
李紅林%高美華%鄭雲會%楊士軍%劉豔鞦%陸衛平
리홍림%고미화%정운회%양사군%류염추%륙위평
Graves病%细胞因子%白介素17%转化生化因子β1%发病机制
Graves病%細胞因子%白介素17%轉化生化因子β1%髮病機製
Graves병%세포인자%백개소17%전화생화인자β1%발병궤제
Graves′disease%Cytokine%Interleukin 17%Transforming growth-beta%Pathogensis
目的：通过检测初发Graves病（ GD）患者的IFN-γ、IL-6、IL-17和TGF-β1等4种细胞因子水平，研究Graves病的发病机制与细胞因子的关系。方法：对23例初发Graves病（ GD组）的患者和23名健康对照者（ NC组）的血清分别用ELISA法检测IFN-γ、IL-6、IL-17和TGF-β1浓度，用电化学发光法检测FT3、FT4和TSH水平，用放射受体分析法测定TrAb水平。结果：GD组与NC组之间年龄、性别均无统计学差异（ t＝0.3348，P＞0.05；χ2＝0.4107，P＞0.05）。 GD组IFN-γ、IL-6、IL-17和TGF-β1的浓度显著高于NC组，差异有统计学意义（ P均＜0.05）；4种细胞因子与FT3、FT4均呈正相关（ r＝值依次为：0.3246、0.4532、0.4310、0.4638；0.4132、0.4415、0.4362、0.4671； P 均＜0.05）；与 TSH 均呈负相关（ r＝值依次为－0.4588、－0.4828、－0.4451、-0.4930；P均＜0.05）。结论：IFN-γ、IL-6、IL-17和TGF-β1等4种细胞因子在初发GD中均高表达；可能共同参与了GD的发病过程，可作为GD免疫功能紊乱的评价指标。
目的：通過檢測初髮Graves病（ GD）患者的IFN-γ、IL-6、IL-17和TGF-β1等4種細胞因子水平，研究Graves病的髮病機製與細胞因子的關繫。方法：對23例初髮Graves病（ GD組）的患者和23名健康對照者（ NC組）的血清分彆用ELISA法檢測IFN-γ、IL-6、IL-17和TGF-β1濃度，用電化學髮光法檢測FT3、FT4和TSH水平，用放射受體分析法測定TrAb水平。結果：GD組與NC組之間年齡、性彆均無統計學差異（ t＝0.3348，P＞0.05；χ2＝0.4107，P＞0.05）。 GD組IFN-γ、IL-6、IL-17和TGF-β1的濃度顯著高于NC組，差異有統計學意義（ P均＜0.05）；4種細胞因子與FT3、FT4均呈正相關（ r＝值依次為：0.3246、0.4532、0.4310、0.4638；0.4132、0.4415、0.4362、0.4671； P 均＜0.05）；與 TSH 均呈負相關（ r＝值依次為－0.4588、－0.4828、－0.4451、-0.4930；P均＜0.05）。結論：IFN-γ、IL-6、IL-17和TGF-β1等4種細胞因子在初髮GD中均高錶達；可能共同參與瞭GD的髮病過程，可作為GD免疫功能紊亂的評價指標。
목적：통과검측초발Graves병（ GD）환자적IFN-γ、IL-6、IL-17화TGF-β1등4충세포인자수평，연구Graves병적발병궤제여세포인자적관계。방법：대23례초발Graves병（ GD조）적환자화23명건강대조자（ NC조）적혈청분별용ELISA법검측IFN-γ、IL-6、IL-17화TGF-β1농도，용전화학발광법검측FT3、FT4화TSH수평，용방사수체분석법측정TrAb수평。결과：GD조여NC조지간년령、성별균무통계학차이（ t＝0.3348，P＞0.05；χ2＝0.4107，P＞0.05）。 GD조IFN-γ、IL-6、IL-17화TGF-β1적농도현저고우NC조，차이유통계학의의（ P균＜0.05）；4충세포인자여FT3、FT4균정정상관（ r＝치의차위：0.3246、0.4532、0.4310、0.4638；0.4132、0.4415、0.4362、0.4671； P 균＜0.05）；여 TSH 균정부상관（ r＝치의차위－0.4588、－0.4828、－0.4451、-0.4930；P균＜0.05）。결론：IFN-γ、IL-6、IL-17화TGF-β1등4충세포인자재초발GD중균고표체；가능공동삼여료GD적발병과정，가작위GD면역공능문란적평개지표。
Objective:To explore the role of cytokines in the pathogenesis of Graves′disease(GD),by detecting the levels of IFN-γ,IL-6,IL-17 and TGF-β1 in GD patients who were newly diagnosed.Methods:A total of 23 patients with new onset GD and 23 gender-and age-matched healthy controls were examined.The levels of serum IFN-γ, IL-6, IL-17 and TGF-β1 were measured by ELISA,FT3,FT4 and TSH levels were determined by ECL IA;TrAb levels were tested by RRA.Results: There were no significant difference among GD and NC group in sex and age match ( t=0.334 8 ,P>0.05;χ2=0.410 7 ,P>0.05 ).The levels of serum IFN-γ,IL-6,IL-17 and TGF-β1 in the GD group were significantly higher than the control group ( P<0.05 ) .Correlation analysis revealed that IFN-γ,IL-6,IL-17 and TGF-β1 were positively correlated with FT3,FT4(r=0.324 6,0.453 2,0.431 0,0.463 8;0.413 2,0.441 5, 0.436 2,0.467 1;P<0.05 ).Conclusion: IFN-γ, IL-6, IL-17 and TGF-β1 are highly expressed in the newly diagnosed GD patients.They play an important role in the pathogenesis of GD ,and provide helpful evaluation indices of immune dysfunction to Graves disease.
