CAJ | 학술논문

目的：研究美沙拉秦、蒙脱石散、酪酸梭菌对2，4，6三硝基苯磺酸（TNBS）／乙醇法诱导的溃疡性结肠炎大鼠IL-17、IL-23、TGF-β1、IFN-γ的影响。方法：将85只大鼠随机分为模型组、美沙拉秦组、蒙脱石散组、酪酸梭菌组、美沙拉秦联合蒙脱石散组5组，每组15只；正常大鼠为空白对照组10只，一共6组。通过TNBS／乙醇法建立溃疡性结肠炎的大鼠模型。按大鼠体表面积折算药量，分别给予相应剂量生理盐水、美沙拉秦、蒙脱石散、酪酸梭菌、美沙拉秦联合蒙脱石散治疗。12 d后处死全部大鼠，收集大鼠的血标本及结肠标本。采用ELISA法测定血清中IL-17、IL-23、TGF-β1、IFN-γ的含量，分别对比6组细胞因子的水平。结果：（1）通过TNBS／乙醇法建立大鼠模型，HE染色病理切片发现大鼠结肠远端炎症明显。（2）模型组、美沙拉秦组、蒙脱石散组、酪酸梭菌组、美沙拉秦联合蒙脱石散组较空白对照组的血清IL-17、IL-23和IFN-γ含量均升高，血清TGF-β1含量均降低，差异有统计学意义（ P＜0.05）。（ 3 ）与模型组比较，蒙脱石散组、酪酸梭菌组、美沙拉秦组的血清IL-17、IL-23和IFN-γ含量均降低，血清TGF-β1含量均升高，差异有统计学意义（P＜0.05）。（4）美沙拉秦、蒙脱石散、酪酸梭菌和美沙拉秦联合蒙脱石散的治疗效果比较，美沙拉秦和美沙拉秦联合蒙脱石散疗效较好，差异有统计学意义（P＜0.05），酪酸梭菌与蒙脱石散疗效差异不明显。结论：（1）从症状体征及病理学检查表明：TNBS／乙醇法成功建立了大鼠溃疡性结肠炎模型。（2）在TNBS／乙醇法诱导的实验性溃疡性结肠炎大鼠血清中IL-17、IL-23、IFN-γ表达增高，与炎症呈正相关；结肠炎大鼠血清中TGF-β1表达降低，与炎症呈负相关。（3）美沙拉秦、蒙脱石散、酪酸梭菌、美沙拉秦联合蒙脱石散可能通过下调模型大鼠促炎因子IL-17、IL-23和调节因子IFN-γ的分泌，上调抗炎因子TGF-β1的分泌，缓解大鼠炎症症状，利于大鼠溃疡性结肠炎的修复。目的：研究美沙拉秦、矇脫石散、酪痠梭菌對2，4，6三硝基苯磺痠（TNBS）／乙醇法誘導的潰瘍性結腸炎大鼠IL-17、IL-23、TGF-β1、IFN-γ的影響。方法：將85隻大鼠隨機分為模型組、美沙拉秦組、矇脫石散組、酪痠梭菌組、美沙拉秦聯閤矇脫石散組5組，每組15隻；正常大鼠為空白對照組10隻，一共6組。通過TNBS／乙醇法建立潰瘍性結腸炎的大鼠模型。按大鼠體錶麵積摺算藥量，分彆給予相應劑量生理鹽水、美沙拉秦、矇脫石散、酪痠梭菌、美沙拉秦聯閤矇脫石散治療。12 d後處死全部大鼠，收集大鼠的血標本及結腸標本。採用ELISA法測定血清中IL-17、IL-23、TGF-β1、IFN-γ的含量，分彆對比6組細胞因子的水平。結果：（1）通過TNBS／乙醇法建立大鼠模型，HE染色病理切片髮現大鼠結腸遠耑炎癥明顯。（2）模型組、美沙拉秦組、矇脫石散組、酪痠梭菌組、美沙拉秦聯閤矇脫石散組較空白對照組的血清IL-17、IL-23和IFN-γ含量均升高，血清TGF-β1含量均降低，差異有統計學意義（ P＜0.05）。（ 3 ）與模型組比較，矇脫石散組、酪痠梭菌組、美沙拉秦組的血清IL-17、IL-23和IFN-γ含量均降低，血清TGF-β1含量均升高，差異有統計學意義（P＜0.05）。（4）美沙拉秦、矇脫石散、酪痠梭菌和美沙拉秦聯閤矇脫石散的治療效果比較，美沙拉秦和美沙拉秦聯閤矇脫石散療效較好，差異有統計學意義（P＜0.05），酪痠梭菌與矇脫石散療效差異不明顯。結論：（1）從癥狀體徵及病理學檢查錶明：TNBS／乙醇法成功建立瞭大鼠潰瘍性結腸炎模型。（2）在TNBS／乙醇法誘導的實驗性潰瘍性結腸炎大鼠血清中IL-17、IL-23、IFN-γ錶達增高，與炎癥呈正相關；結腸炎大鼠血清中TGF-β1錶達降低，與炎癥呈負相關。（3）美沙拉秦、矇脫石散、酪痠梭菌、美沙拉秦聯閤矇脫石散可能通過下調模型大鼠促炎因子IL-17、IL-23和調節因子IFN-γ的分泌，上調抗炎因子TGF-β1的分泌，緩解大鼠炎癥癥狀，利于大鼠潰瘍性結腸炎的脩複。
목적：연구미사랍진、몽탈석산、락산사균대2，4，6삼초기분광산（TNBS）／을순법유도적궤양성결장염대서IL-17、IL-23、TGF-β1、IFN-γ적영향。방법：장85지대서수궤분위모형조、미사랍진조、몽탈석산조、락산사균조、미사랍진연합몽탈석산조5조，매조15지；정상대서위공백대조조10지，일공6조。통과TNBS／을순법건립궤양성결장염적대서모형。안대서체표면적절산약량，분별급여상응제량생리염수、미사랍진、몽탈석산、락산사균、미사랍진연합몽탈석산치료。12 d후처사전부대서，수집대서적혈표본급결장표본。채용ELISA법측정혈청중IL-17、IL-23、TGF-β1、IFN-γ적함량，분별대비6조세포인자적수평。결과：（1）통과TNBS／을순법건립대서모형，HE염색병리절편발현대서결장원단염증명현。（2）모형조、미사랍진조、몽탈석산조、락산사균조、미사랍진연합몽탈석산조교공백대조조적혈청IL-17、IL-23화IFN-γ함량균승고，혈청TGF-β1함량균강저，차이유통계학의의（ P＜0.05）。（ 3 ）여모형조비교，몽탈석산조、락산사균조、미사랍진조적혈청IL-17、IL-23화IFN-γ함량균강저，혈청TGF-β1함량균승고，차이유통계학의의（P＜0.05）。（4）미사랍진、몽탈석산、락산사균화미사랍진연합몽탈석산적치료효과비교，미사랍진화미사랍진연합몽탈석산료효교호，차이유통계학의의（P＜0.05），락산사균여몽탈석산료효차이불명현。결론：（1）종증상체정급병이학검사표명：TNBS／을순법성공건립료대서궤양성결장염모형。（2）재TNBS／을순법유도적실험성궤양성결장염대서혈청중IL-17、IL-23、IFN-γ표체증고，여염증정정상관；결장염대서혈청중TGF-β1표체강저，여염증정부상관。（3）미사랍진、몽탈석산、락산사균、미사랍진연합몽탈석산가능통과하조모형대서촉염인자IL-17、IL-23화조절인자IFN-γ적분비，상조항염인자TGF-β1적분비，완해대서염증증상，리우대서궤양성결장염적수복。
Objective:To establish the model of rats with UC by 2,4,6 trinitrobenzene sulfonic acid ( TNBS)/ethanol to understand the changes of IL-17,IL-23,TGF-β1,IFN-γin pathogenesis of ulcerative colitis (UC) and the impacts of mesalazine,mont-morillonite powder and clostridium on that.Methods:85 rats were divided randomly into 6 groups including the model ,mesalazine, montmorillonite,clostridium,mesalazine combined with montmorillonite powder group (15 rats per group ) and an additional control group of 10 normal rats.TNBS/ethanol was used to establish the model of rats with UC.The dosage of model, mesalazine, montmorillonite powder ,clostridium and mesalazine combined with montmorillonite powder were converted by surface area of rat and given to the rats by gastric-tube daily.All rats were executed at the 12th day of the molding and treatment ,and the blood and colon samples were collected.The content of IL-17,IL-23,TGF-β1,IFN-γwas measured in the blood by the ELISA method.The different changes of the four cytokines were compared separately.Results: ( 1 ) TNBS/ethanol was used to establish the model of rats with UC.The obvious inflammation can be observed on distal colon of rats by pathologic sections of HE stained .(2) The levels of plasma IL-17,IL-23 and IFN-γin the model,mesalazine,montmorillonite,clostridium and mesalazine combined with montmorillonite powder group were higher than ones in the control gruop (P<0.05),meanwhile,the levels of plasma TGF-β1 are lower (P<0.05).3.Comparing with the model group,the levels of plasma IL-17,IL-23 and IFN-γin the mesalazine,montmorillonite,clostridium and mesalazine combined with montmorillonite powder group were lower , and the levels of TGF-β1 were higer.The differences have statistic significative ( P<0.05).(4)Comparing the efficacy of treatment among mesalazine ,montmorillonite powder,clostridium and mesalazine combined with montmorillonite powder ,mesalazine and mesalazine combined with montmorillonite powder gets the best results ( P<0.05 ).The efficacy between montmorillonite powder and clostridium is similar.Conclusion:(1) TNBS/ethanol can be used to establish the model of rats with UC and can be successfully approved by the measurement of symptoms and pathologic investigation .(2)The IL-17,IL-23 and IFN-γare very active higher in the blood of rats with experimental ulcerative colitis induced by TNBS /ethanol, and have a positive correlation with inflammation.The TGF-β1 is in decrease in the blood of rats with colitis , and has a Negative Correlation with inflammation.(3)Mesalazine,montmorillonite powder,clostridium and mesalazine combined with montmorillonite powder may renovate the damage of inflammatory tissues of rats with experimental ulcerative colitis , and relieve the symptom of inflammation by reducing exudation of proinflammatory cytokines IL-17, IL-23 and immune regulatory cytokines IFN-γby producing the exudation of proinflammatory cytokines TGF-β1 to be good for recovery of UC.