CAJ | 학술논문

目的：了解MAGE-E1 mRNA在胶质瘤中的表达，探讨将它用于胶质瘤免疫治疗的可能性。方法：建立实时定量PCR方法，检测47例人胶质瘤及14例正常脑组织中MAGE-E1，管家基因HPRT1作为内参，以MAGE-E1／HPRT1值计算MAGE-E1 mRNA表达量，并对MAGE-E1 mRNA表达与临床指标的关系进行分析。结果：在正常脑组织及胶质瘤中， MAGE-E1高度表达率分别为7.1％和66.0％，两者比较具有统计学差异（ P＜0.05）。 MAGE-E1 mRNA的表达与病人性别、年龄、病理类型和级别无关。结论：MAGE-E1 mRNA在胶质瘤中水平较高，提示MAGE-E1可能成为胶质瘤免疫治疗的候选靶抗原。
목적：료해MAGE-E1 mRNA재효질류중적표체，탐토장타용우효질류면역치료적가능성。방법：건립실시정량PCR방법，검측47례인효질류급14례정상뇌조직중MAGE-E1，관가기인HPRT1작위내삼，이MAGE-E1／HPRT1치계산MAGE-E1 mRNA표체량，병대MAGE-E1 mRNA표체여림상지표적관계진행분석。결과：재정상뇌조직급효질류중， MAGE-E1고도표체솔분별위7.1％화66.0％，량자비교구유통계학차이（ P＜0.05）。 MAGE-E1 mRNA적표체여병인성별、년령、병리류형화급별무관。결론：MAGE-E1 mRNA재효질류중수평교고，제시MAGE-E1가능성위효질류면역치료적후선파항원。
Objective:To investigate the quantitative expression of Melanoma-associated antigen MAGE-E1 mRNA in glioma, and explore its potential for immunotherapy in glioma.Methods:To establish a quantitative real-time polymerase chain reaction ( qRT-PCR) method to quantitatively determine MAGE-E1 mRNA in glioma.A total of 47 human glioma and 14 normal brain tissue specimens were analyzed.MAGE-E1 mRNA was normalized to HPRT1, a housekeeping gene.MAGE-E1/HPRT1 was used to evaluate the expression level of MAGE-E1 mRNA.Results:High-level expression of MAGE-E1 was observed in 7.1%of normal brain and 66.0%glioma tissues,which shown significant difference with P<0.05.There was irrelevant between the expression of MAGE-E1 mRNA and clinicopathogical parameters ,such as gender ,age,histological subtype and grade.Conclusion:With higher expression of MAGE-E1 in glioma tissues,it might be a potential target for immunotherapy of glioma.