中国卒中杂志
中國卒中雜誌
중국졸중잡지
CHINESE JOURNAL OF STROKE
2015年
2期
107-112
,共6页
楠迪%郭淮莲%何其华%宋江曼
楠迪%郭淮蓮%何其華%宋江曼
남적%곽회련%하기화%송강만
慢性脑缺血%激光共聚焦显微镜%毛细血管%细动脉%细静脉
慢性腦缺血%激光共聚焦顯微鏡%毛細血管%細動脈%細靜脈
만성뇌결혈%격광공취초현미경%모세혈관%세동맥%세정맥
Chronic cerebral hypoperfusion%Confocol microscopy%Capillary%Arterioles%Venules
目的通过建立慢性脑缺血模型，活体动态观察慢性脑缺血后小鼠脑血管形态学变化。
<br> 方法 Tie2-GFP转基因小鼠7只，左侧颈总动脉永久性结扎结合右侧颈总动脉短暂结扎方法制备慢性脑缺血模型，分别在脑缺血前（D0)和脑缺血后7 d（D7），利用颅窗和激光共聚焦显微镜活体观察小鼠脑缺血前后皮层毛细血管、细动脉及细静脉的变化情况。
<br> 结果①缺血前和脑缺血后7 d毛细血管直径分别是（6.62±0.75）μm和（12.50±3.29）μm，脑缺血后毛细血管直径较缺血前扩张（P＜0.001）；②脑缺血前和脑缺血后7 d毛细血管密度分别为每感兴趣区域（region of interest，ROI）（11.67±1.72）个和每ROI（11.08±2.06）个，差异无显著性（P=0.0583）；③脑缺血前和脑缺血后7 d分支毛细血管、网状毛细血管、集合毛细血管及细动静脉直通支直径分别为（6.33±0.94）μmvs （12.36±3.20）μm，（6.87±1.10）μmvs （12.37±2.78）μm，（6.37±0.52）μmvs （11.41±3.10）μm及（6.35±0.92）μmvs （13.91±6.17）μm，脑缺血后均扩张（P分别为＜0.01，＜0.01，＜0.01及＜0.05），其中细动静脉直通支扩张最显著；④细静脉直径在D0和D7时分别为（19.59±8.74）μm和（24.81±6.25）μm，脑缺血后细静脉显著扩张（P=0.0054）；而细动脉直径在D0和D7时分别是（12.71±2.10）μm和（13.20±3.09）μm，无显著性改变（P=0.2947）；⑤可见细动静脉迂曲、吻合支开放及毛细血管走行改变等血管重构现象。
<br> 结论慢性脑缺血后皮层血管发生毛细血管扩张、细静脉扩张等改变，这些改变可能有助于调节脑血流量。
目的通過建立慢性腦缺血模型，活體動態觀察慢性腦缺血後小鼠腦血管形態學變化。
<br> 方法 Tie2-GFP轉基因小鼠7隻，左側頸總動脈永久性結扎結閤右側頸總動脈短暫結扎方法製備慢性腦缺血模型，分彆在腦缺血前（D0)和腦缺血後7 d（D7），利用顱窗和激光共聚焦顯微鏡活體觀察小鼠腦缺血前後皮層毛細血管、細動脈及細靜脈的變化情況。
<br> 結果①缺血前和腦缺血後7 d毛細血管直徑分彆是（6.62±0.75）μm和（12.50±3.29）μm，腦缺血後毛細血管直徑較缺血前擴張（P＜0.001）；②腦缺血前和腦缺血後7 d毛細血管密度分彆為每感興趣區域（region of interest，ROI）（11.67±1.72）箇和每ROI（11.08±2.06）箇，差異無顯著性（P=0.0583）；③腦缺血前和腦缺血後7 d分支毛細血管、網狀毛細血管、集閤毛細血管及細動靜脈直通支直徑分彆為（6.33±0.94）μmvs （12.36±3.20）μm，（6.87±1.10）μmvs （12.37±2.78）μm，（6.37±0.52）μmvs （11.41±3.10）μm及（6.35±0.92）μmvs （13.91±6.17）μm，腦缺血後均擴張（P分彆為＜0.01，＜0.01，＜0.01及＜0.05），其中細動靜脈直通支擴張最顯著；④細靜脈直徑在D0和D7時分彆為（19.59±8.74）μm和（24.81±6.25）μm，腦缺血後細靜脈顯著擴張（P=0.0054）；而細動脈直徑在D0和D7時分彆是（12.71±2.10）μm和（13.20±3.09）μm，無顯著性改變（P=0.2947）；⑤可見細動靜脈迂麯、吻閤支開放及毛細血管走行改變等血管重構現象。
<br> 結論慢性腦缺血後皮層血管髮生毛細血管擴張、細靜脈擴張等改變，這些改變可能有助于調節腦血流量。
목적통과건립만성뇌결혈모형，활체동태관찰만성뇌결혈후소서뇌혈관형태학변화。
<br> 방법 Tie2-GFP전기인소서7지，좌측경총동맥영구성결찰결합우측경총동맥단잠결찰방법제비만성뇌결혈모형，분별재뇌결혈전（D0)화뇌결혈후7 d（D7），이용로창화격광공취초현미경활체관찰소서뇌결혈전후피층모세혈관、세동맥급세정맥적변화정황。
<br> 결과①결혈전화뇌결혈후7 d모세혈관직경분별시（6.62±0.75）μm화（12.50±3.29）μm，뇌결혈후모세혈관직경교결혈전확장（P＜0.001）；②뇌결혈전화뇌결혈후7 d모세혈관밀도분별위매감흥취구역（region of interest，ROI）（11.67±1.72）개화매ROI（11.08±2.06）개，차이무현저성（P=0.0583）；③뇌결혈전화뇌결혈후7 d분지모세혈관、망상모세혈관、집합모세혈관급세동정맥직통지직경분별위（6.33±0.94）μmvs （12.36±3.20）μm，（6.87±1.10）μmvs （12.37±2.78）μm，（6.37±0.52）μmvs （11.41±3.10）μm급（6.35±0.92）μmvs （13.91±6.17）μm，뇌결혈후균확장（P분별위＜0.01，＜0.01，＜0.01급＜0.05），기중세동정맥직통지확장최현저；④세정맥직경재D0화D7시분별위（19.59±8.74）μm화（24.81±6.25）μm，뇌결혈후세정맥현저확장（P=0.0054）；이세동맥직경재D0화D7시분별시（12.71±2.10）μm화（13.20±3.09）μm，무현저성개변（P=0.2947）；⑤가견세동정맥우곡、문합지개방급모세혈관주행개변등혈관중구현상。
<br> 결론만성뇌결혈후피층혈관발생모세혈관확장、세정맥확장등개변，저사개변가능유조우조절뇌혈류량。
Objective To dynamically observe the morphological changes of the cortical vessel in mice after chronic cerebral hypoperfusion through a cranial window.
<br> Methods The left common carotid artery ligation and transient right common carotid artery ligation operations were performed on 7 Tie2-GFP transgenic mice. The cortical vessels were observed using confocol microscopy through a cranial window before brain ischemia (D0) and 7 days after brain ischemia (D7). The diameter and density of capillaries, along with the diameter and morphological change of arterioles and venules, were analyzed.
<br> Results①The diameters of capillary before and 7 days after the brain ischemia were (6.62±0.75)μm and (12.50±3.29)μm, which increased significantly after brain ischemia (P<0.001).②The capillary densities before and 7 days after the brain ischemia were (11.67±1.72) segments per region of interest (ROI) and (11.08±2.06) segments per ROI. The difference was not signiifcant (P=0.0583).③The diameters of the precapillary arteriole, post capillary venule, true capillary and thoroughfare channel before and 7 days after the brain ischemia were (6.33±0.94)μmvs (12.36±3.20)μm, (6.87±1.10)μmvs (12.37±2.78)μm, (6.37±0.52)μmvs (11.41±3.10)μm and (6.35±0.92)μm vs (13.91±6.17)μm, respectively, all increased significantly after brain ischemia (P<0.01, <0.01,<0.01, and <0.05, respectively). The most signiifcant dilation was observed in thoroughfare channel.④The diameters of venules on D0 and D7 were (19.59±8.74)μm and (24.81±6.25)μm, which increased signiifcantly after brain ischemia (P=0.0054). However, the diameters of arterioles on D0 and D7 were (12.71±2.10)μm and (13.20±3.09)μm, the difference was not signiifcant (P=0.2947);⑤Capillary remodeling, tortuosity and new anastomosis between venules were observed after brain ischemia.
<br> Conclusion The dilation of capillaries and venules were observed after brain ischemia. These changes may contribute to the regulation of cerebral blood flow after chronic cerebral hypoperfusion.
