中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2015年
2期
189-192
,共4页
郑兵%谢芳一%蔡国辉%朱如彩%李柯%高守泉%谭电波%郝晓勇%秦裕辉
鄭兵%謝芳一%蔡國輝%硃如綵%李柯%高守泉%譚電波%郝曉勇%秦裕輝
정병%사방일%채국휘%주여채%리가%고수천%담전파%학효용%진유휘
雪峰虫草%树突细胞%杀伤细胞%HepG-2细胞
雪峰蟲草%樹突細胞%殺傷細胞%HepG-2細胞
설봉충초%수돌세포%살상세포%HepG-2세포
Ophiocordyceps xuefengensis%Dendritic cells%Killer cells%HepG-2 cells
目的:研究雪峰虫草对DC-CIK细胞增殖及肝癌HepG-2细胞杀伤作用。方法:常规分离健康人外周血单个核细胞并诱导生成DC细胞和CIK细胞,将DC细胞与CIK细胞按1∶5共培养7 d后给药组加入不同浓度的雪峰虫草水提取物,第10天观察形态并计数各组DC-CIK细胞;收集培养第10天的DC-CIK细胞作为效应细胞,对数生长期HepG-2肝癌细胞作为靶细胞,使HepG-2∶DC-CIK靶效比为1∶5,cck-8法检测DC-CIK对HepG-2的杀伤率。结果:雪峰虫草水提物组对DC-CIK有显著的促增长作用,其作用的最佳浓度为0.1 mg/ml。雪峰虫草诱导的DC-CIK细胞对肝癌HepG-2细胞的杀伤作用优于常规方法培养的DC-CIK细胞;常规方法培养的DC-CIK细胞加雪峰虫草与常规方法培养的DC-CIK细胞对肝癌HepG-2细胞的杀伤作用无显著性差异,雪峰虫草体外直接杀伤肝癌HepG-2细胞的作用不明显。结论:雪峰虫草通过促进 DC-CIK细胞增殖而增强其杀伤肝癌HepG-2细胞的作用。
目的:研究雪峰蟲草對DC-CIK細胞增殖及肝癌HepG-2細胞殺傷作用。方法:常規分離健康人外週血單箇覈細胞併誘導生成DC細胞和CIK細胞,將DC細胞與CIK細胞按1∶5共培養7 d後給藥組加入不同濃度的雪峰蟲草水提取物,第10天觀察形態併計數各組DC-CIK細胞;收集培養第10天的DC-CIK細胞作為效應細胞,對數生長期HepG-2肝癌細胞作為靶細胞,使HepG-2∶DC-CIK靶效比為1∶5,cck-8法檢測DC-CIK對HepG-2的殺傷率。結果:雪峰蟲草水提物組對DC-CIK有顯著的促增長作用,其作用的最佳濃度為0.1 mg/ml。雪峰蟲草誘導的DC-CIK細胞對肝癌HepG-2細胞的殺傷作用優于常規方法培養的DC-CIK細胞;常規方法培養的DC-CIK細胞加雪峰蟲草與常規方法培養的DC-CIK細胞對肝癌HepG-2細胞的殺傷作用無顯著性差異,雪峰蟲草體外直接殺傷肝癌HepG-2細胞的作用不明顯。結論:雪峰蟲草通過促進 DC-CIK細胞增殖而增彊其殺傷肝癌HepG-2細胞的作用。
목적:연구설봉충초대DC-CIK세포증식급간암HepG-2세포살상작용。방법:상규분리건강인외주혈단개핵세포병유도생성DC세포화CIK세포,장DC세포여CIK세포안1∶5공배양7 d후급약조가입불동농도적설봉충초수제취물,제10천관찰형태병계수각조DC-CIK세포;수집배양제10천적DC-CIK세포작위효응세포,대수생장기HepG-2간암세포작위파세포,사HepG-2∶DC-CIK파효비위1∶5,cck-8법검측DC-CIK대HepG-2적살상솔。결과:설봉충초수제물조대DC-CIK유현저적촉증장작용,기작용적최가농도위0.1 mg/ml。설봉충초유도적DC-CIK세포대간암HepG-2세포적살상작용우우상규방법배양적DC-CIK세포;상규방법배양적DC-CIK세포가설봉충초여상규방법배양적DC-CIK세포대간암HepG-2세포적살상작용무현저성차이,설봉충초체외직접살상간암HepG-2세포적작용불명현。결론:설봉충초통과촉진 DC-CIK세포증식이증강기살상간암HepG-2세포적작용。
Objective:To study the effects of Ophiocordyceps xuefengensis on proliferation of DC -CIK cells and the activity of killing HepG-2 cells by DC-CIK cells.Methods:Peripheral blood mononuclear cells were routinely isolated and induced into DC and CIK.DC and CIK co-cultured by 1∶5 for 7 days,then Ophiocordyceps xuefengensis were added into medicine group ,observed the mor-phology of the cells on the tenth day and counted the DC-CIK number of each group.DC-CIK cells acted as effector cells and the HepG-2 cells as target cells , cck-8 method for the detection of DC-CIK in the killing rate of HepG-2.Results: The Ophiocordyceps xuefengensis was able to proliferate the DC-CIK dramatically ,the optimal concentration was 0.1 mg/ml.Cultivation of Ophiocordyceps xuefengensis induced DC-CIK cells on HepG-2 cells killing effect was better than that of the routine method of DC-CIK cells; the effection of Ophiocordyceps xuefengensis killing HepG-2 cells was not obviously.Conclusion: Ophiocordyceps xuefengensis can enhance the anti-tumor activity of DC-CIK mainly by promoting the proliferation of it.