CAJ | 학술논문

建立了蓝莓和黑莓中VC含量的高效液相色谱测定方法。样品用0.5%草酸溶液低温振荡提取后，进行高效液相色谱分析，色谱柱为 Agilent C18（4.6 mm×250 mm，5 m），流动相为甲醇-0.1%磷酸溶液（5∶95），流速0.8 mL/min，检测波长242 nm。研究结果表明，VC在0.02 mg/mL~0.4 mg/mL范围内与峰面积呈良好的线性关系，r为0.9996，平均加样回收率为97.1%，RSD均小于3.0%。所建立方法快速简便、准确度高，能够用于测定蓝莓和黑莓中VC的含量。
건립료람매화흑매중VC함량적고효액상색보측정방법。양품용0.5%초산용액저온진탕제취후，진행고효액상색보분석，색보주위 Agilent C18（4.6 mm×250 mm，5 m），류동상위갑순-0.1%린산용액（5∶95），류속0.8 mL/min，검측파장242 nm。연구결과표명，VC재0.02 mg/mL~0.4 mg/mL범위내여봉면적정량호적선성관계，r위0.9996，평균가양회수솔위97.1%，RSD균소우3.0%。소건립방법쾌속간편、준학도고，능구용우측정람매화흑매중VC적함량。
To develop a method for the determination of Vitamin C in blueberry and blackberry.An HPLC method has been developed to determine Vitamin C on Agilent C18 (4.6 mm×250 mm,5 m) column with the mobile phase of consisting of 5 %(v/v) methanol and 0.1%phosphoric acid.The flow rate was 0.8 mL/min and the UV detection wavelength was set at 242 nm.The result showed that linearity of Vitamin C was good in the ranges of 0.02 mg/mL-0.4 mg/mL(r=0.999 6).The average recovery of Vitamin C was 97.1%with RSD less than 3.0%.The method is sample and accurate and could be used to control the quality of blueberry and blackberry.