CAJ | 학술논문

采用流动相（乙腈-磷酸盐缓冲液，PH=2.3）为稀释液，建立了样品直接稀释、高效液相色谱（HPLC）分离、二极管阵列检测器（PDA）检测，同时快速测定鲜橙中的酒石酸、VC、乳酸、乙酸、柠檬酸、琥珀酸、苹果酸的可靠方法。该方法以保留时间（Rt）定性，外标法定量；7种组分在0.02 mg/mL~3.75 mg/mL的浓度范围内，标准曲线有良好的线性关系，相关系数大于0.9995；样品在0.2、0.5、5.0 g/kg 3个加标水平的平均回收率为92%~104%（n=10），相对标准偏差为1.1%~2.9%；方法的定性检出限（LOD）为0.02 g/kg~0.05 g/kg、定量检出限（LOQ）为0.05 g/kg~0.2 g/kg。
채용류동상（을정-린산염완충액，PH=2.3）위희석액，건립료양품직접희석、고효액상색보（HPLC）분리、이겁관진렬검측기（PDA）검측，동시쾌속측정선등중적주석산、VC、유산、을산、저몽산、호박산、평과산적가고방법。해방법이보류시간（Rt）정성，외표법정량；7충조분재0.02 mg/mL~3.75 mg/mL적농도범위내，표준곡선유량호적선성관계，상관계수대우0.9995；양품재0.2、0.5、5.0 g/kg 3개가표수평적평균회수솔위92%~104%（n=10），상대표준편차위1.1%~2.9%；방법적정성검출한（LOD）위0.02 g/kg~0.05 g/kg、정량검출한（LOQ）위0.05 g/kg~0.2 g/kg。
A method was developed for simultaneous determination of tartaric acid, vitamin C, lactic acid, acetic acid, citric acid, succinic acid, and malic acid in fresh orange. The sample was diluted directly by mobile phase (acetonitrile-NaH2PO4 buffer solution,pH=2.3), and then analyzed by high-performance liquid chromatography (HPLC) with diode array detector (PDA). The method for qualitative analysis was based on retention time. And external standard method was used for calculation. Good linear relationship was achieved in the range of 0.02 mg/mL-3.75 mg/mL for the 7 components with correlation coefficients above 0.999 5. The recoveries of the method ranged from 92%to 104%(n=10) with the relative standard deviations (RSDs) among 1.1%-2.9%at 3 spiked levels of 0.2, 0.5, 5.0 g/kg. The limit of detection (LOD) of this method is among 0.02 g/kg-0.05 g/kg for the 7 components, while the limit of quantification (LOQ) was 0.05 g/kg-0.2 g/kg for the 7 components. This is a simple, rapid, sensitive method for detection of organic acid and VC.