CAJ | 학술논문

根据香草醛-高氯酸显色反应后样品吸光度的差异，用Box-Behnken响应面法优化了海马中甾醇的提取条件。主要确定了提取温度、提取时间及料液比3个因素对甾醇提取效果的影响。并以胆固醇为标准品，测定了海马中总甾醇的含量。结果表明：提取温度为79℃，提取时间为7.7 h，料液比为80 ml/g时，海马中的总甾醇提取量最高，提取量为5.17 mg/g。通过高效液相分析，初步分离海马总甾醇提取物，为海马中甾醇成分的提取和分离提供了初步的依据。
근거향초철-고록산현색반응후양품흡광도적차이，용Box-Behnken향응면법우화료해마중치순적제취조건。주요학정료제취온도、제취시간급료액비3개인소대치순제취효과적영향。병이담고순위표준품，측정료해마중총치순적함량。결과표명：제취온도위79℃，제취시간위7.7 h，료액비위80 ml/g시，해마중적총치순제취량최고，제취량위5.17 mg/g。통과고효액상분석，초보분리해마총치순제취물，위해마중치순성분적제취화분리제공료초보적의거。
In the present work, we optimized the extracting conditions of total sterol in Hippocampus kuda by Box-Behnken response surface method according to the difference in absorbance of vanillin perchloric acid color reaction. Three factors associated with extraction effect were determined , including extraction temperature, extraction time and ratio of material to liquid. The content of total sterol in the hippocampus was also determined with a standard of cholesterol. The results showed that the highest total sterol extraction amount was obtained to 5.17 mg/g when the extraction temperature was at 79℃, extraction time at 7.7 h, and the ratio of solid to liquid at 80 mL/g. The purified sample was further identified by high performance liquid chromatography (HPLC). This paper provided a preliminary basis for the extraction and separation of the sterol composition in Hippocampus kuda.